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Deoxythymidine 5'-Triphosphate, [Methyl-3H]-, Aqueous Sol, Tetrasodium Salt, Specific Activity: 70-90 Ci (2.59-3.33 TBq)/mMole, 1 mCi (37 MBq)

dTTP, tritiated on the methyl group. This product is supplied in aqueous solution. dTTP that is tritiated on the methyl ground will incorporate labeled thymidine into DNA via DNA polymerase.

貨號
產品尺寸
NET221A250UC
250 µCi
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NET221A001MC
1 mCi
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NET221A005MC
5 mCi
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詳細信息

Deoxythymidine triphosphate (dTTP), labeled on the methyl group with 3H. Tritiated dTTP is commonly used to make probes for in situ hybridization studies via nick translation, or to measure nucleotide incorporation via reverse transcriptase or DNA polymerase activity.

References:

  1. Fonatsch, C., Gradl, G., Kolbus, U. et al. Hum Genet (1990) Chromosomal in situ hybridization of a Hodgkin's disease-derived cell line (L540) using DNA probes for TCRA, TCRB, MET, and rRNA, Human Genetics, 84:427.
  2. White, E.L., Parker, W.B., Macy, L.J., Shaddix, S.C., McCaleb, G., Secrist, J.A., Vince, R., Shannon, W.M. (1989) Comparison of the effect of Carbovir, AZT, and dideoxynucleoside triphosphates on the activity of human immunodeficiency virus reverse transcriptase and selected human polymerases, Biochemical and Biophysical Research Communications, 161(2):393-398.

規格

標簽位置 Specifically Labeled
分子量 570.6
核苷酸 dTTP
產品品牌名稱 NEN Radiochemicals
放射性同位素 H-3
放射性核素 3H
運輸條件 幹冰
特殊訂購信息 This is a radioactive product - shipping address must have a license to receive radioactive materials.
產品尺寸 1 mCi
資源
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產品手冊

Radiometric Reagents Guide 2010-2011

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