BioQule NGS System

Easy to Use, Compact Solution for Automating Low Throughput Library Preparation and Quantitation

• Full walkaway automation (80% reduction in HOT)
• Easy to use (only 4 touch points)
• Avoid human error (>99% pass rate)
• Open system able to automate different applications

The BioQule^™ NGS System provides a simplified, robust walk-away solution for NGS library preparation. This system consists of a NGS preparation and quantitation instrument, cassette, and a reagent plate to generate NGS libraries. The BioQule NGS System uses an intuitive interface, automated thermal cycling, an on-board magnet, and integrated quality control through optical quantification to produce reliable NGS libraries from as little as 10 ng of starting material. The BioQule NGS System is the ideal for a lab of any size performing NGS library preparation and can easily be used by novice users to automation or NGS sample preparation.

• Construct DNA-seq libraries from as little as 10 ng input
• 8 libraries constructed and quantitated per run
• Save 5 hours of hands-on time
  

  The BioQule NGS System features:

  The BioQule NGS System has been specifically designed so no automation experience is required to generate NGS libraries. Simply pipette your samples, IPA, beads, and two enzymes into the plate, place the cartridge and plate in the instrument, close the door and walk-away.

  This solution creates 8 NGS libraries from as little as 10 ng of starting material per run while saving 4.5 hours, on average, of hands-on time during library preparation.

  The BioQule NGS System has been designed so no automation experience is required to prepare NGS libraries. Simply prepare your plate, place the cartridge and plate into the instrument, close the door and start the instrument.

  

  Figure 1. Placing reagent plate in instrument

  

  Figure 2. Placing cartridge in instrument

  Library Preparation & Quality Control in the Same System

  The BioQule NGS System is equipped with automated fluorescence-based optical quantitation to measure the concentration of each library. You will no longer need to move your samples from one instrument to another to perform concentration analysis on your libraries.

  

  The BioQule NGS System constructs libraries with size distributions that are equivalent to those prepared manually.

  

  A Library Prepared Manually

  

  A Library Prepared with the BioQule NGS System

  
   

  The BioQule NGS System Compatible Kits*:

• NEXTFLEX^® Rapid DNA-Seq kit 2.0
• NEXTFLEX^® Rapid XP V2 DNA-Seq kit

  * More kits in development. Please inquire.
   

  Simplified Reagent & Cartridge Preparation

  Each run of the BioQule NGS System uses one reagent plate and one cartridge. The reagent plate comes pre-plated with most reagents required for NGS library preparation. Once one run is complete, simply change out the reagent plate and cartridge and you can construct another 8 NGS libraries.

  
  • Easy-to-use interface
  • Automated thermal cycling
  • On-board magnet
  • Integrated quality control through optical quantification
  • Easy-to-use consumable plates and cartridges
  • Easy NGS library preparation