Columns - Liquid Chromatography

The 10 mm guard cartridge holder provides protection against compounds that irreversibly absorb onto the stationary phase. It also provides the ultimate in column protection with a selectable filter frit for elimination of particulates along with a guard cartridge packed with a selectable stationary phase to protection against compounds that irreversibly absorb onto the stationary phase.

GreenSep^™ Diol is designed specifically for SFC with a high-density diol surface coverage which ensures separations are better and more reproducible than conventional unbonded silica. GreenSep Diol is particularly suitable for acidic and basic analytes. This phase provides the selectivity of silica, without its reactivity.

Epic^™ HILIC Silica is based on ultrapure silica rigorously sized to produce highly efficient columns. Epic HILIC silica is pretreated to yield uniform distribution of silanol sites essential for reproducible HILIC chromatography. Epic HILIC silica is useful for the separation of polar bases which can ion exchange with silanols enabling the retention of these polar bases.

GreenSep^™ Pyridyl Amide stationary phase is the first column of choice when developing an SFC separation and is excellent overall for the separation of acids, amines and neutrals without additives. GreenSep Pyridyl Amide provides flexibility for the SFC chromatographer with mobile phase composition and fraction collection being greatly simplified without the use of amino additives.

Epic^™ Polar is a high density C18 packing specifically engineered for the retention of polar analytes and allows full interaction with the bonded hydrocarbon phase, even in 100% aqueous mobile phases. Under these highly aqueous conditions, our novel proprietary bonding chemistry allows the bonded chains to remain fully extended in the mobile phase. Epic Polar can retain highly water-soluble compounds such as small organic acids, water-soluble vitamins, purines and pyrimidines, cathecholamines and other polar compounds.

The Epic^™ PFP LB (low bleed) is a pentafluorophenyl that has been baseline stabilized and is ready for high performance separations. The Epic PFP LB is a truly unique stationary phase with properties significantly different than C18 phases. This unique characteristic results from bonded pentafluorophenyl groups imparting a π-π electron interaction which produces an enhanced retention for many compounds, such as natural products, halogenated compounds, aromatics, conjugated compounds and trace impurities in complex matrices. Many of these high-performance separations were not possible with existing PFP columns especially in the area of trace impurities where baseline bleed levels were unacceptable. Epic PFP LB columns have been stabilized to provide low column bleed, increased lifetimes, better pH stability, and superior LC-MS performance.

Epic^™ HILIC POH (POH for polyhydroxylated) is a new stationary phase for HILIC chromatography. It is composed of a polyhydroxylated polymer coated and bound to silica. This polymer coating enhances the behavior of the stationary phase under HILIC operating conditions. This composition provides hydroxyl levels that are well above conventional hydroxyl and diol type stationary phases.

The Epic^™ FO LB is a baseline stabilized alkyl perfluorinated C8 (perfluorooctyl), with low bleed characteristics and is well suited to the separation of trace impurities, especially for halogenated analytes, lipophilic compounds and environmental samples. Fluorinated phases are able to perform many unique and difficult separations which cannot be performed on the best available C18 columns. Traditionally, fluorinated phases have suffered from poor column lifetimes, unstable baselines and column bleed, especially when used with mass spectrometry (MS). Epic FO LB has been stabilized to provide low column bleed, increased lifetimes, better pH stability, and superior LC-MS performance.

Epic^™ Biphenyl is a truly unique stationary phase with properties significantly different than ODS phases. This unique character results from bonded biphenyl groups, covalently attached to high purity silica, imparting a π-π electron interaction which produces an enhanced retention for many compounds particularly those that contain polarizable electrons. Many classes of compounds contain polarizable electrons including halogenated compounds, aromatics, nitro aromatics and conjugated systems.

Epic^™ Amine HD (high density) is a polymeric amino phase bonded to silica. Applications include HILIC, weak acids, and sugars. The Epic bonding technology produces columns of superior performance and durability. These performance characteristics result from the high bonding density found in the Epic bonding process.

GreenSep^™ Ethyl Pyridine II is based on ethyl pyridine chemistry, providing a unique character for this stationary phase. GreenSep Ethyl Pyridine II is ideally suited for the retention and rapid separation of chemicals containing acid groups without additives. This phase is non-endcapped and provides superior separation of acids in comparison with the GreenSep Ethyl Pyridine. GreenSep Ethyl Pyridine II can easily replace conventional stationary phases used in SFC and deliver excellent performance.

GreenSep^™ Naphthyl is a naphthalene based SFC material, with high bonding density and intrinsic base deactivation due to a rigid structure that also enables the shape selectivity needed for many diastereomeric separations. It exhibits strong π-π interaction and charge transfer interactions, performing well for diastereomer separations and non-polar compounds. The unique properties of GreenSep Naphthyl place its selectivity between graphitized carbon and alkyl type stationary phases.

The StarRise^™ line shows equivalent selectivity to the Waters SunFire^™ and has a long column lifetime and excellent reproducibility. The StarRise C18 provides equivalent selectivity to the SunFire C18. StarRise columns provide symmetrical peaks for improved resolution and quantization of acidic neutral and basic compounds a low and intermediate pH range. It is available in many particle sizes as well as preparative bulk. Other StarRise phases are also available including C8.

GreenSep^™ Ethyl Pyridine is an endcapped phase and has proven superior to conventional stationary phases (such as diol, cyano etc.) in the areas of separation selectivity, peak shape and loading capacity. The chromatogram shown (right) is a prime example of the superior peak shape performance obtainable with the GreenSep Ethyl Pyridine column with SFC. Mobile phase composition and fraction collection is greatly simplified without the use of amino additives. GreenSep Ethyl Pyridine provides better separation for amines in comparison with the GreenSep Ethyl Pyridine II.

GreenSep^™ Pyridyl Amide stationary phase is the first column of choice when developing an SFC separation and is excellent overall for the separation of acids, amines and neutrals without additives. GreenSep Pyridyl Amide provides flexibility for the SFC chromatographer with mobile phase composition and fraction collection being greatly simplified without the use of amino additives.

GreenSep^™ PFP is a fluorinated aromatic stationary phase providing a highly selective character for SFC separations. It is specifically designed for the separation of geometrical isomers as well as diastereomers. GreenSep PFP is the column of choice in separating compounds that contain aromatic groups, polarizable electrons and conjugate systems. In addition, it is useful for the separation of halogenated compounds. In many cases GreenSep PFP provides orthogonal separations when compared to GreenSep Nitro. GreenSep PFP can easily replace conventional stationary phases used in SFC and deliver superior performance.

GreenSep^™ Nitro SFC stationary phase is nitro aromatic based and has proven superior to conventional stationary phases (such as diol, cyano etc.) in the areas of separation selectivity and loading capacity. GreenSep Nitro provides unique selectivity and is specifically designed for the separation of geometrical isomers as well as diastereomers. It is the column of choice in separating compounds that contain an aromatic group, polarizable electrons, halogenated groups and conjugate systems.

Epic^™ Polar is a high density C18 packing specifically engineered for the retention of polar analytes and allows full interaction with the bonded hydrocarbon phase, even in 100% aqueous mobile phases. Under these highly aqueous conditions, our novel proprietary bonding chemistry allows the bonded chains to remain fully extended in the mobile phase. Epic Polar can retain highly water-soluble compounds such as small organic acids, water-soluble vitamins, purines and pyrimidines, cathecholamines and other polar compounds.

Epic^™ Phenyl-Hexyl employs a 6-carbon (hexyl) linked phenyl phase bonded to high purity silica, where the hexyl alkyl chain delivers unique selectivity and increased hydrolytic stability when compared to propyl-linked phenyl phase chemistry. This retentive phase provides different selectivity to straight chain hydrocarbon phases like C6, C8 or C18, and it is especially useful for aromatic analytes, complex samples and polar pharmaceutical compounds. The Epic high-density bonding technology delivers to the chromatographer superior performance, durability, and enhanced lot-to-lot reproducibility. Epic Phenyl-Hexyl offers the chromatographer a truly superior phenyl-based interaction for enhanced chromatographic performance.

The Epic^™ Phenyl phase is π-basic (electron donating) and is similar in overall retention to alkyl phases. The alternate selectivity exhibited by phenyl phases is explained by the π-π interactions available through the phenyl ring. Compounds that exhibit this alternate selectivity on the Epic Phenyl phase include antibiotics such as tetracycline, moderate bases such as anesthetics, some acidic compounds such as quinoline antibiotics and nucleosides. The Epic high-density bonding technology delivers superior performance, durability and enhanced lot-to-lot reproducibility. Epic Phenyl offers a truly superior phenyl-based interaction for enhanced chromatographic performance.

The Epic^™ PFP LB (low bleed) is a pentafluorophenyl that has been baseline stabilized and is ready for high performance separations. The Epic PFP LB is a truly unique stationary phase with properties significantly different than C18 phases. This unique characteristic results from bonded pentafluorophenyl groups imparting a π-π electron interaction which produces an enhanced retention for many compounds, such as natural products, halogenated compounds, aromatics, conjugated compounds and trace impurities in complex matrices. Many of these high-performance separations were not possible with existing PFP columns especially in the area of trace impurities where baseline bleed levels were unacceptable. Epic PFP LB columns have been stabilized to provide low column bleed, increased lifetimes, better pH stability, and superior LC-MS performance.

Epic^™ Naphthyl is a naphthalene based material, with high bonding density and intrinsic base deactivation due to a rigid structure that also enables the shape selectivity needed for many diastereomeric separations. It exhibits strong π-π interactions and charge transfer interactions and performs well for diastereomer separations and non-polar compounds. The unique properties of Epic Naphthyl places its selectivity between graphitized carbon and alkyl type stationary phases.

Epic^™ HILIC POH (POH for polyhydroxylated) is a new stationary phase for HILIC chromatography. It is composed of a polyhydroxylated polymer coated and bound to silica. This polymer coating enhances the behavior of the stationary phase under HILIC operating conditions. This composition provides hydroxyl levels that are well above conventional hydroxyl and diol type stationary phases.

Epic^™ HILIC PI is a new stationary phase for HILIC chromatography. It consists of an aromatic amine based stationary phase bound to silica. This composition provides for excellent retention and peak shape for polar amine compounds and superior retention of acids. Many of the commercially available HILIC stationary phases are converted normal phase columns. These normal phase columns yield poor methods, poor separations and lack durability. Epic HILIC PI is specifically designed for HILIC chromatography and can achieve high performance separations, yield rugged methods and deliver long column lifetimes.

Epic^™ HILIC FL is useful for retention and separation of polar and non polar compounds that are not retained or separated on conventional reversed phase columns. It consists of a fluorinated based stationary phase bound to silica. This composition provides for excellent retention and peak shape for polar halogenated, polar amines and polar aromatic compounds. Epic HILIC FL is specifically designed for HILIC chromatography and can achieve high performance separations, yield rugged methods and deliver long column lifetimes.