Enzymology

L-Histidine, labeled with ¹⁴C at the 1,2 positions. L-Histidine is commonly used in amino acid uptake and metabolic studies. This is a replacement for NEC277E050UC.

The AlphaLISA^® SureFire^® Ultra™ p-SHP-2 (Tyr580) assay is a sandwich immunoassay for quantitative detection of phospho-SHP-2 (phosphorylated on Tyr580) in cellular lysates using Alpha Technology.

The AlphaLISA^® SureFire^® Ultra™ total SHP-2 assay is a sandwich immunoassay for quantitative detection of SHP-2 (phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be a normalization or control assay in conjunction with the AlphaLISA SureFire phospho-SHP-2 assay.

This kit has been pre-validated for use in combination with Pelago Bioscience’s CETSA^® (Cellular Thermal Shift Assay) technology. Please note that further assay optimization will be required in order to adapt the kit to your particular cell line and experimental conditions. A valid CETSA^® licence from Pelago Bioscience or a single kit agreement is required for using the CETSA^® technology.

The AlphaLISA^® SureFire^® Ultra™ total p70 S6K assay is a sandwich immunoassay for quantitative detection of p70 S6K (phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be a normalization or control assay in conjunction with the AlphaLISA SureFire phospho-p70 S6K assay.

This kit has been pre-validated for use in combination with Pelago Bioscience’s CETSA^® (Cellular Thermal Shift Assay) technology. Please note that further assay optimization will be required in order to adapt the kit to your particular cell line and experimental conditions. A valid CETSA^® licence from Pelago Bioscience or a single kit agreement is required for using the CETSA^® technology.

The AlphaLISA^® SureFire^® Ultra™ total NFκB assay is a sandwich immunoassay for quantitative detection of NFκB (phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be a normalization or control assay in conjunction with the AlphaLISA SureFire phospho-NFκB assay.

The AlphaLISA^® SureFire^® Ultra™ total EGFR assay is a sandwich immunoassay for quantitative detection of EGFR (phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be a normalization or control assay in conjunction with the AlphaLISA SureFire phospho-EGFR assay.

The AlphaLISA^® SureFire^® Ultra™ total Akt1/2/3 assay is a sandwich immunoassay for quantitative detection of Akt1/2/3 (phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be a normalization or control assay in conjunction with the AlphaLISA SureFire phospho-Akt1/2/3 assay.

This kit has been pre-validated for use in combination with Pelago Bioscience’s CETSA^® (Cellular Thermal Shift Assay) technology. Please note that further assay optimization will be required in order to adapt the kit to your particular cell line and experimental conditions. A valid CETSA^® licence from Pelago Bioscience or a single kit agreement is required for using the CETSA^® technology.

The AlphaLISA^® SureFire^® Ultra™ p-SHP-2 (Tyr542) assay is a sandwich immunoassay for quantitative detection of phospho-SHP-2 (phosphorylated on Tyr542) in cellular lysates using Alpha Technology.

The AlphaLISA^® SureFire^® Ultra™ p-SHP-1 (Tyr564) assay is a sandwich immunoassay for quantitative detection of phospho-SHP-1 (phosphorylated on Tyr564) in cellular lysates using Alpha Technology.

The AlphaLISA^® SureFire^® Ultra™ p-SHP-1 (Tyr536) assay is a sandwich immunoassay for quantitative detection of phospho-SHP-1 (phosphorylated on Tyr536) in cellular lysates using Alpha Technology.

The AlphaLISA^® SureFire^® Ultra™ p-EGFR (Tyr1068) assay is a sandwich immunoassay for quantitative detection of phospho-EGFR (phosphorylated on Tyr1068) in cellular lysates using Alpha Technology.

The AlphaLISA^® SureFire^® Ultra™ total eIF2α assay is a sandwich immunoassay for quantitative detection of eIF2α (phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be a normalization or control assay in conjunction with the AlphaLISA SureFire phospho-eIF2α assay.

This kit has been pre-validated for use in combination with Pelago Bioscience’s CETSA^® (Cellular Thermal Shift Assay) technology. Please note that further assay optimization will be required in order to adapt the kit to your particular cell line and experimental conditions. A valid CETSA^® licence from Pelago Bioscience or a single kit agreement is required for using the CETSA^® technology.

LANCE Ultra phospho-TBK1 (Ser172) kits are designed for the detection of phosphorylated TBK1 in cell lysates using a simple, homogeneous LANCE Ultra assay (no wash steps). This assay is compatible with both adherent and suspension cells.

LANCE Ultra phospho-STAT1 (Tyr701) kits are designed for the detection of phosphorylated STAT1 in cell lysates using a simple, homogeneous LANCE Ultra assay (no wash steps). This assay is compatible with both adherent and suspension cells.

LANCE Ultra total STAT1 kits are designed for the detection of STAT1 (both phosphorylated and non-phosphorylated) in cell lysates using a simple, homogeneous LANCE Ultra TR-FRET assay (no wash steps). This assay is compatible with both adherent and suspension cells, and is intended to be used as a control or normalization assay in conjunction with the LANCE phospho-STAT1 assay.

Generic peptide substrate for use in TR-FRET biochemical kinase assays. Refer to Selector Guide for validated kinases. ULight^™-labeled synthetic peptide derived from residues 73-85 of rat Acetyl-CoA Carboxylase in which Ser77 is mutated to Ala (also known as SAMS). The ULight-Acetyl-CoA Carboxylase peptide can be phosphorylated at Ser 79 by the AMPKα1 kinase. Peptide sequence : CHMRSAMSGLHLVKRR. Please note this product replaces TRF0118-D or TRF0118-M, now being supplied lyophilized rather than in liquid (frozen) format.

LANCE Ultra phospho-AMPKα (Thr172) kits are designed for the detection of phosphorylated AMPKα in cell lysates using a simple, homogeneous LANCE Ultra assay (no wash steps). This assay is compatible with both adherent and suspension cells.

LANCE Ultra phospho-LCK (Tyr394) kits are designed for the detection of phosphorylated LCK in cell lysates using a simple, homogeneous LANCE Ultra assay (no wash steps). This assay is compatible with both adherent and suspension cells.

LANCE Ultra phospho-ZAP-70 (Tyr319) kits are designed for the detection of phosphorylated ZAP-70 in cell lysates using a simple, homogeneous LANCE Ultra assay (no wash steps). This assay is compatible with both adherent and suspension cells.

LANCE Ultra Total eIF2α kits are designed for the detection of total eIF2α (phosphorylated and non-phosphorylated) in cell lysates using a simple, homogeneous LANCE Ultra assay (no wash steps). This assay can be used as a normalization assay for phospho-eIF2α detection, and is compatible with both adherent and suspension cells.

LANCE Ultra Total BTK kits are designed for the detection of total BTK (phosphorylated and non-phosphorylated) in cell lysates using a simple, homogeneous LANCE Ultra assay (no wash steps). This assay can be used as a normalization assay for phospho-BTK detection, and is compatible with both adherent and suspension cells.

LANCE Ultra phospho-BTK (Tyr223) kits are designed for the detection of phosphorylated BTK in cell lysates using a simple, homogeneous LANCE Ultra assay (no wash steps). This assay is compatible with both adherent and suspension cells.

LANCE Ultra total NFκB kits are designed for the detection of NFκB (both phosphorylated and non-phosphorylated) in cell lysates using a simple, homogeneous LANCE Ultra TR-FRET assay (no wash steps). This assay is compatible with both adherent and suspension cells, and is intended to be used as a control or normalization assay in conjunction with the LANCE phospho-NFκB assay.

LANCE Ultra phospho-NFκB (Ser536) kits are designed for the detection of phosphorylated NFκB in cell lysates using a simple, homogeneous LANCE Ultra TR-FRET assay (no wash steps). This assay is compatible with both adherent and suspension cells.

The AlphaLISA^® SureFire^® Ultra™ total MKK4 assay is a sandwich immunoassay for quantitative detection of MKK4 (phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be a normalization or control assay in conjunction with the AlphaLISA SureFire phospho-MKK4 assay.

This kit has been pre-validated for use in combination with Pelago Bioscience’s CETSA^® (Cellular Thermal Shift Assay) technology. Please note that further assay optimization will be required in order to adapt the kit to your particular cell line and experimental conditions. A valid CETSA^® licence from Pelago Bioscience or a single kit agreement is required for using the CETSA^® technology.