Many SFC separations have utilized “older normal phase HPLC types” of stationary phases such as unmodified silica, diol, amino and cyano. These phases are poorly adapted to SFC and present a number of limitations for SFC separations including low capacity, poor selectivity and poor peak shape. GreenSep™ stationary phases, on the other hand, have been specifically engineered for SFC separations, paying close attention to bonding coverage, density and all factors leading to high capacity phases which exhibit excellent selectivity and peak shape.
The GreenSep range features a variety of selectivities offering orthogonality. All of these materials are available in analytical and also semi-preparative (10 mm), and preparative (20 mm, 30 mm and 50 mm i.d.) dimensions. Additionally, comprehensive technical and method development assistance is offered. Shown below is a column selection guide for the development of a SFC separation with the GreenSep Pyridyl Amide being the go-to column of choice, followed by the other columns.
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The 10 mm guard cartridge holder provides protection against compounds that irreversibly absorb onto the stationary phase. It also provides the ultimate in column protection with a selectable filter frit for elimination of particulates along with a guard cartridge packed with a selectable stationary phase to protection against compounds that irreversibly absorb onto the stationary phase.
GreenSep™ Diol is designed specifically for SFC with a high-density diol surface coverage which ensures separations are better and more reproducible than conventional unbonded silica. GreenSep Diol is particularly suitable for acidic and basic analytes. This phase provides the selectivity of silica, without its reactivity.
GreenSep™ Pyridyl Amide stationary phase is the first column of choice when developing an SFC separation and is excellent overall for the separation of acids, amines and neutrals without additives. GreenSep Pyridyl Amide provides flexibility for the SFC chromatographer with mobile phase composition and fraction collection being greatly simplified without the use of amino additives.
GreenSep™ Ethyl Pyridine II is based on ethyl pyridine chemistry, providing a unique character for this stationary phase. GreenSep Ethyl Pyridine II is ideally suited for the retention and rapid separation of chemicals containing acid groups without additives. This phase is non-endcapped and provides superior separation of acids in comparison with the GreenSep Ethyl Pyridine. GreenSep Ethyl Pyridine II can easily replace conventional stationary phases used in SFC and deliver excellent performance.
GreenSep™ Naphthyl is a naphthalene based SFC material, with high bonding density and intrinsic base deactivation due to a rigid structure that also enables the shape selectivity needed for many diastereomeric separations. It exhibits strong π-π interaction and charge transfer interactions, performing well for diastereomer separations and non-polar compounds. The unique properties of GreenSep Naphthyl place its selectivity between graphitized carbon and alkyl type stationary phases.
GreenSep™ Ethyl Pyridine is an endcapped phase and has proven superior to conventional stationary phases (such as diol, cyano etc.) in the areas of separation selectivity, peak shape and loading capacity. The chromatogram shown (right) is a prime example of the superior peak shape performance obtainable with the GreenSep Ethyl Pyridine column with SFC. Mobile phase composition and fraction collection is greatly simplified without the use of amino additives. GreenSep Ethyl Pyridine provides better separation for amines in comparison with the GreenSep Ethyl Pyridine II.
GreenSep™ Pyridyl Amide stationary phase is the first column of choice when developing an SFC separation and is excellent overall for the separation of acids, amines and neutrals without additives. GreenSep Pyridyl Amide provides flexibility for the SFC chromatographer with mobile phase composition and fraction collection being greatly simplified without the use of amino additives.
GreenSep™ PFP is a fluorinated aromatic stationary phase providing a highly selective character for SFC separations. It is specifically designed for the separation of geometrical isomers as well as diastereomers. GreenSep PFP is the column of choice in separating compounds that contain aromatic groups, polarizable electrons and conjugate systems. In addition, it is useful for the separation of halogenated compounds. In many cases GreenSep PFP provides orthogonal separations when compared to GreenSep Nitro. GreenSep PFP can easily replace conventional stationary phases used in SFC and deliver superior performance.
GreenSep™ Nitro SFC stationary phase is nitro aromatic based and has proven superior to conventional stationary phases (such as diol, cyano etc.) in the areas of separation selectivity and loading capacity. GreenSep Nitro provides unique selectivity and is specifically designed for the separation of geometrical isomers as well as diastereomers. It is the column of choice in separating compounds that contain an aromatic group, polarizable electrons, halogenated groups and conjugate systems.
GreenSep™ PFP is a fluorinated aromatic stationary phase providing a highly selective character for SFC separations. It is specifically designed for the separation of geometrical isomers as well as diastereomers. GreenSep PFP is the column of choice in separating compounds that contain aromatic groups, polarizable electrons and conjugate systems. In addition, it is useful for the separation of halogenated compounds. In many cases GreenSep PFP provides orthogonal separations when compared to GreenSep Nitro. GreenSep PFP can easily replace conventional stationary phases used in SFC and deliver superior performance.
GreenSep™ Nitro SFC stationary phase is nitro aromatic based and has proven superior to conventional stationary phases (such as diol, cyano etc.) in the areas of separation selectivity and loading capacity. GreenSep Nitro provides unique selectivity and is specifically designed for the separation of geometrical isomers as well as diastereomers. It is the column of choice in separating compounds that contain an aromatic group, polarizable electrons, halogenated groups and conjugate systems.
GreenSep™ Ethyl Pyridine is an endcapped phase and has proven superior to conventional stationary phases (such as diol, cyano etc.) in the areas of separation selectivity, peak shape and loading capacity. The chromatogram shown (right) is a prime example of the superior peak shape performance obtainable with the GreenSep Ethyl Pyridine column with SFC. Mobile phase composition and fraction collection is greatly simplified without the use of amino additives. GreenSep Ethyl Pyridine provides better separation for amines in comparison with the GreenSep Ethyl Pyridine II.
GreenSep™ Pyridyl Amide stationary phase is the first column of choice when developing an SFC separation and is excellent overall for the separation of acids, amines and neutrals without additives. GreenSep Pyridyl Amide provides flexibility for the SFC chromatographer with mobile phase composition and fraction collection being greatly simplified without the use of amino additives.
GreenSep™ DEAP is a diethyl amino propyl stationary phase with greater selectivity and superior peak shapes to conventional amino phases. GreenSep DEAP can enable the chromatographer to use simple mobile phases, reducing the need for additives and leading to easier fraction collection. It is particularly useful for alcohols and amides. GreenSep DEAP can easily replace conventional stationary phases used in SFC and deliver superior performance.
GreenSep™ Basic is based on imidazole chemistry providing a highly basic character for this stationary phase. GreenSep Basic offers the chromatographer greater flexibility in developing separations and is the SFC column ideally suited for the retention and rapid separation of chemicals containing amine groups. GreenSep Basic is the primary column of choice for the retention and rapid separation of compounds containing strong amine groups, without use of additives. GreenSep Basic can easily replace conventional stationary phases used in SFC and deliver superior performance.
GreenSep™ Silica has been developed specifically for SFC use. GreenSep Silica is metal free ultra-high purity chromatographic media that is pressure stable and specifically engineered for high performance SFC separations. The surface is treated to produce maximum SFC separation interactions and loading capacity while maintaining superior peak shape performance for many pharmaceutical compounds. GreenSep Silica can perform separation of chemicals with superior peak shapes than typical HPLC silica columns.
GreenSep™ 4-Ethyl Pyridine II is based on ethyl pyridine chemistry and is non-endcapped, providing a unique character for this stationary phase. GreenSep 4-Ethyl Pyridine II is the SFC column ideally suited for the retention and rapid separation of chemicals containing acid groups. GreenSep 4-Ethyl Pyridine II can easily replace conventional stationary phases used in SFC and deliver superior performance. This phase provides alternative selectivity to the GreenSep Ethyl Pyridine II. This phase is non-endcapped and provides superior separation of acids in comparison with the GreenSep 4-Ethyl Pyridine.
GreenSep™ 4-Ethyl Pyridine is an alternative to and provides different selectivity to GreenSep Ethyl Pyridine (2-ethyl pyridine). This endcapped stationary phase has proven superior to conventional stationary phases (such as diol and cyano phases) in the areas of separation selectivity, peak shape and loading capacity. GreenSep 4-Ethyl Pyridine can easily replace conventional stationary phases used in SFC while delivering superior performance. GreenSep 4-Ethyl Pyridine provides better separations for amines in comparison with the 4-Ethyl Pyridine II.
GreenSep™ Ethyl Pyridine II is based on ethyl pyridine chemistry, providing a unique character for this stationary phase. GreenSep Ethyl Pyridine II is ideally suited for the retention and rapid separation of chemicals containing acid groups without additives. This phase is non-endcapped and provides superior separation of acids in comparison with the GreenSep Ethyl Pyridine. GreenSep Ethyl Pyridine II can easily replace conventional stationary phases used in SFC and deliver excellent performance.
GreenSep™ Ethyl Pyridine is an endcapped phase and has proven superior to conventional stationary phases (such as diol, cyano etc.) in the areas of separation selectivity, peak shape and loading capacity. The chromatogram shown (right) is a prime example of the superior peak shape performance obtainable with the GreenSep Ethyl Pyridine column with SFC. Mobile phase composition and fraction collection is greatly simplified without the use of amino additives. GreenSep Ethyl Pyridine provides better separation for amines in comparison with the GreenSep Ethyl Pyridine II.
GreenSep™ Pyridyl Amide stationary phase is the first column of choice when developing an SFC separation and is excellent overall for the separation of acids, amines and neutrals without additives. GreenSep Pyridyl Amide provides flexibility for the SFC chromatographer with mobile phase composition and fraction collection being greatly simplified without the use of amino additives.
GreenSep™ PFP is a fluorinated aromatic stationary phase providing a highly selective character for SFC separations. It is specifically designed for the separation of geometrical isomers as well as diastereomers. GreenSep PFP is the column of choice in separating compounds that contain aromatic groups, polarizable electrons and conjugate systems. In addition, it is useful for the separation of halogenated compounds. In many cases GreenSep PFP provides orthogonal separations when compared to GreenSep Nitro. GreenSep PFP can easily replace conventional stationary phases used in SFC and deliver superior performance.
GreenSep™ Nitro SFC stationary phase is nitro aromatic based and has proven superior to conventional stationary phases (such as diol, cyano etc.) in the areas of separation selectivity and loading capacity. GreenSep Nitro provides unique selectivity and is specifically designed for the separation of geometrical isomers as well as diastereomers. It is the column of choice in separating compounds that contain an aromatic group, polarizable electrons, halogenated groups and conjugate systems.
GreenSep™ Naphthyl is a naphthalene based SFC material, with high bonding density and intrinsic base deactivation due to a rigid structure that also enables the shape selectivity needed for many diastereomeric separations. It exhibits strong π-π interaction and charge transfer interactions, performing well for diastereomer separations and non-polar compounds. The unique properties of GreenSep Naphthyl place its selectivity between graphitized carbon and alkyl type stationary phases.
GreenSep™ FluoroBasic is based on fluorinated imidazole chemistry, providing a highly basic and fluorinated character for this stationary phase. A fluorophilic retention mechanism can be particular useful in medicinal chemistry and drug discovery, where more than a third of newly approved small molecule drugs contain fluorine. GreenSep FluoroBasic is ideally suited for the retention and rapid separation of chemicals containing amine and acidic groups. These columns can easily replace conventional stationary phases used in SFC and deliver superior performance.
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