How to use Growdex hydrogels
We offer a range of products for scalable animal-free 3D cell culture applications, providing convenience and consistent results. GrowDex® hydrogels are natural nanofibers of cellulose extracted from wood and sourced from sustainable and responsibly managed forests. Supplied as ready-to-use, prepacked syringes, GrowDex mimics the extracellular matrix and supports cell growth and differentiation. These animal-free matrices are a proven solution for automation and high throughput screening. They can be used for 3D cell culture for spheroids and organoids, in personalised medicine, regenerative medicine, organ-on-a-chip models, drug release studies and more.
Cells can be recovered from GrowDex for downstream processing, with a one-step enzymatic process using our GrowDase™.
Benefits of GrowDex hydrogels:
Why choose animal-free hydrogels for cell culture?
Despite being widely used, animal derived matrices are often poorly defined, contain contaminating molecules (RNA/DNA), suffer from lot-to-lot variability, and can be difficult to use. With animal free hydrogels, like GrowDex, you can benefit from:
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GrowDase™ enzyme breaks down GrowDex® hydrogel to form a solution in an easy one- step efficient cell recovery process. 3D cell structures such as spheroids, organoid or biopsies are retained with no impact on cell viability or functionality. Liberated cells can be used in gene or protein expression studies or for future experiments.
GrowDex®-A is a ready to use, transparent hydrogel that has been specifically developed to bind different biotinylated molecules, whose presence may enhance cell culture conditions for specific 3D applications. GrowDex-A consists of avidin conjugated nanofibrillar cellulose which can be customized by binding different biotinylated molecules e.g. proteins or peptides, to create a cell specific matrix. Ambient handling and shear thinning properties of GrowDex-A enable easy dispensing and use in automated systems.
GrowDex®-T is an animal-free ready to use hydrogel that has been specifically developed to offer superior imaging properties, but without compromising on any of the advantages of the original hydrogel GrowDex. GrowDex-T can be mixed directly with cells and culture media, no gelation or cross-linking step required. Ambient handling and shear thinning properties of GrowDex-T enable easy dispensing and use in automated systems.
GrowDex® is an animal-free hydrogel that enables complete control over the culture environment for cells.
GrowDex can be used in a wide range of cell-based assays such as microscopic imaging or metabolic activity measurement. GrowDex has been validated on various cell models and applications including primary hepatocytes, patient derived cells, 3D organoid formation, hepatocyte toxicity, stem cell proliferation and differentiation, tumour cell migration and invasion, neurite outgrowth and network formation.
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