High-Content Analysis (HCA) or High-Content Screening (HCS) combines high-throughput automated imaging and analysis to extract quantitative multi-parametric data at the single-cell level. Originally developed as a complementary technology to traditional biochemical high-throughput screening (HTS) in drug discovery, today high-content screening is established in a far broader area of the life science space as an unbiased imaging method to assess cellular function.
High-Content Imaging (HCI) is typically defined as lower throughput automated microscopy (<100,000 samples or data points) compared to high-content screening. However, high-content imaging is often used interchangeably with the terms high-content screening and high-content analysis.1
High-content screening offers a number of advantages over traditional techniques:
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Spinning disk confocal microscopy offers several advantages over conventional optical microscopy, including widefield and laser scanning confocal microscopy.
Proprietary automated water-immersion objectives with very high numerical aperture deliver and capture more photons and provide a higher resolution in XYZ than conventional objectives.
Stability, brightness and spectral fit for excitation are key considerations for illumination sources in fluorescent imaging.
The large-format sCMOS camera – one in Operetta CLS or up to four of them in the Opera Phenix - delivers low noise, wide dynamic range, and high resolution – perfect for sensitive and quantitative measurements at short exposure times.
More accurately target your object of interest for significantly reduced acquisition and analysis times with intelligent acquisition technology for HCS.
Machine learning techniques enable supervised and unsupervised feature selection and clustering of highly multi-parametric data sets.
Operetta® CLS™ is a high throughput microplate imager for high-content analysis (HCA). It can acquire, analyze and manage fluorescence, brightfield and digital phase contrast images.
The Opera Phenix® Plus High-Content Screening System is the premier confocal solution for today's most demanding high content applications.
Enable sensitive imaging on our cellular vivo imaging systems -- at whatever resolution you desire. With our custom-designed optics, including optical filters, objectives and zoom lenses, you have the ideal view for any application.
Read how scientists around the globe are using our high-content screening products and services to advance their research in a variety of fields.
