Improved Mycotoxin Analysis Performance
Mycotoxins are secondary metabolites produced by fungi under favorable climatic conditions. They present immunotoxic, carcinogenic and hepatotoxic characteristics making them highly dangerous when consumed at higher levels than legal limits established by the European Union.
Combination of immunoaffinity columns (IAC) with high performance liquid chromatography (HPLC) or Liquid Chromatography with tandem mass spectrometry (LC-MS/MS) is a quantitative method for the detection of mycotoxins in samples such as grains, nuts, feed, dairy and more. Samples are purified by the column and then analyzed with HPLC or LC-MS/MS. Immunoaffinity column chromatography is a great method for mycotoxin analysis due to high sensitivity, selectivity, and ease of use.
While similar levels of analytical performance can be achieved with or without mycotoxin cleanup columns, the immunoaffinity purification does help to show cleaner chromatogram with less interference and background noise, therefore providing better and more reliable testing results. This is due to the exceptionally high selectivity of IAC for mycotoxin analysis.
The IAC uses the highly specific interactions between antigen and antibody to purify and enrich the target substance. The specific antibody present in the column selectively binds the target substance in the sample, and the unbound impurities flow out of the column. The target substance bound on the column is then eluted with eluent, so as to achieve the high-efficiency purification and concentration of the target substance.
Highly concentrated and purified samples enhance the accuracy of the results with minimal to none matrix effects. This is more pronounced in complex sample matrices and increases the sensitivity to meet all regulatory MRLs.
Product Features
- High sensitivity
- High specificity
- Good recovery rate, up to 90-110%
- High-sample loading capacities
- Fast flow rate
- Easy-to-use
- Safe and environmentally friendly
