LANCE® (Lanthanide Chelate Excite) TR-FRET technology combines the benefits of Time Resolved Fluorescence (TRF) with those of Förster Resonance Energy Transfer (FRET) in a simple, highly-sensitive, and highly reproducible assay platform for detection, quantitation and screening in microplate format. We now also offer 爱游戏平台注册登录
Cisbio HTRF® assay technology. HTRF (Homogeneous Time Resolved Technology), allowing us to provide a wide range of assays kits in a homogenous TR-FRET technology.
The use of long-lifetime fluorescent lanthanide chelates, rather than other fluorophores, allows for a delay in measurement between excitation and emissions, which occur simultaneously in standard fluorometric assays. Furthermore, FRET detection utilizes energy donor and energy acceptor fluorophore labels in solution to create a no-wash detection assay. By combining the benefits of TRF and FRET, LANCE TR-FRET offers a multitude of benefits over standard fluorometric detection:
- Homogeneous assay format (no wash steps, no separation steps)
- Reduced background leading to better S:B
- Large Stoke’s shift
- Wider dynamic range
- Extended signal stability
- Increased sensitivity. Lower detection limits of 10 pg/mL compared to 100 pg/mL for standard fluorometric detection
- Faster time to results
From biomarker detection assays, to cAMP/GPCR studies, to kinase and epigenetic assays, our LANCE® and LANCE Ultra TR-FRET assays are sure to save you time and hassle.