Revvity offers a complete suite of cell function reagents and kits for visualizing key cellular events including viability, apoptosis, mitochondrial membrane potential, as well as indicators for calcium, reactive oxygen species (ROS), or hypoxia.
PhenoVue™ ROS Total Oxidative Stress Indicators, encompassing ROS-490, ROS-570 and ROS-670, enable a myriad of applications as well as multiplexing capability to unravel ROS related biological effects.
PhenoVue Fluorescent Calcium Indicators, encompassing Fura-2 AM, Fluo-4 AM, Cal-520 AM, Cal-590 AM, Cal-520 AM Bright and Cal-590 AM Bright, enable a variety of applications as well as multiplexing capability to unravel intracellular calcium mediated functions.
PhenoVue HypoxiTRAK™ is a permeable hypoxia indicator, which exhibits far-red fluorescence. Its spectral characteristics are shared with DRAQ5™.
PhenoVue Live/Dead Cell Viability Assay Kit provides a two-color green and red fluorescence method which enables the simultaneous determination of live and dead cells using two different dyes.
PhenoVue™ Live/Dead Cell Viability Assay Kit enables the simultaneous determination of live and dead cells and is part of Revvity’s portfolio of cellular imaging reagents. Calcein AM dye is a cell permeable fluorescent dye which generates a bright green, fluorescent signal, whereas Propidium Iodide dye enters damaged cell membranes of dead cells and exhibits a bright red fluorescence. This assay kit provides an easy-to-use method for measuring cell proliferation, cell viability, cytotoxicity and apoptosis.
PhenoVue™ HypoxiTRAK™ is a cell-permeable hypoxia indicator and is part of Revvity’s portfolio of cellular imaging reagents. It exhibits far-red fluorescence and is validated for use in imaging microscopy and high-content screening applications. PhenoVue HypoxiTRAK exhibits a maximum excitation wavelength at 647 nm and a maximum emission wavelength of 697 nm.
PhenoVue™ ROS-570, Total Oxidative Stress Indicator is a cell permeable fluorescent reactive oxygen species indicator and is part of Revvity’s portfolio of cellular imaging reagents. PhenoVue ROS-570, Total Oxidative Stress Indicator exhibits orange fluorescence and is validated for use in imaging microscopy and high-content screening applications. It exhibits a maximum excitation wavelength at 574 nm and a maximum emission wavelength of 588 nm.
PhenoVue™ ROS-490, Total Oxidative Stress Indicator is a cell permeable fluorescent reactive oxygen species indicator and is part of Revvity’s portfolio of cellular imaging reagents. PhenoVue ROS-490, Total Oxidative Stress Indicator exhibits green fluorescence and is validated for use in imaging microscopy and high-content screening applications. It exhibits a maximum excitation wavelength at 498 nm and a maximum emission wavelength of 517 nm.
PhenoVue™ ROS-670, Total Oxidative Stress Indicator is a cell permeable fluorescent reactive oxygen species indicator and is part of Revvity’s portfolio of cellular imaging reagents. PhenoVue ROS-670, Total Oxidative Stress Indicator exhibits red fluorescence and is validated for use in imaging microscopy and high-content screening applications. It exhibits a maximum excitation wavelength at 651 nm and a maximum emission wavelength of 670 nm.
PhenoVue™ Fura-2 AM, is a high affinity and permeable fluorescent ratiometric Ca2+ indicator and is part of Revvity’s portfolio of cellular imaging reagents. PhenoVue Fura-2 AM Ca2+ indicator exhibits bright green fluorescence and is validated for use in imaging microscopy and high-content screening applications. It exhibits a maximum excitation wavelength shift from 336 to 365 nm upon binding to free intracellular Ca2+, and a maximum emission wavelength of 505 nm.
PhenoVue™ Fluo-4 AM, is permeable fluorescent Ca2+ indicator and is part of Revvity’s portfolio of cellular imaging reagents. PhenoVue Fluo-4 AM Ca2+ indicator exhibits green fluorescence and is validated for use in imaging microscopy and high-content screening applications. It exhibits a maximum excitation wavelength at 495 nm and a maximum emission wavelength of 528 nm.
PhenoVue™ Cal-590 AM is a cell permeable fluorescent Ca2+ indicator and is part of Revvity’s portfolio of cellular imaging reagents. PhenoVue Cal-590 AM Ca2+ indicator exhibits orange fluorescence and is validated for use in imaging microscopy and high-content screening applications. It exhibits a maximum excitation wavelength at 574 nm and a maximum emission wavelength of 588 nm.
PhenoVue™ Cal-520 AM is a cell permeable fluorescent Ca2+ indicator and is part of Revvity’s portfolio of cellular imaging reagents. PhenoVue Cal-520 AM Ca2+ indicator exhibits green fluorescence and is validated for use in imaging microscopy and high-content screening applications. It exhibits a maximum excitation wavelength at 493 nm and a maximum emission wavelength of 515 nm.
PhenoVue™ Cal-590 AM Bright is a cell permeable fluorescent Ca2+ indicator and is part of Revvity’s portfolio of cellular imaging reagents. PhenoVue Cal-590 AM Bright Ca2+ indicator exhibits orange fluorescence and is validated for use in imaging microscopy and high-content screening applications. It exhibits a maximum excitation wavelength at 581 nm and a maximum emission wavelength of 593 nm.
PhenoVue™ Cal-520 AM Bright is a cell permeable fluorescent Ca2+ indicator and is part of Revvity’s portfolio of cellular imaging reagents. PhenoVue Cal-520 AM Bright Ca2+ indicator exhibits green fluorescence and is validated for use in imaging microscopy and high-content screening applications. It exhibits a maximum excitation wavelength at 493 nm and a maximum emission wavelength of 515 nm.