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For cost-effective automated screening and batch plate processing our luciferin-based reporter gene systems produce a long-lived glow type signal with a half-life of several hours.

Reporter gene assays with easily measurable phenotypes
Characterize the strength of promoters and enhancers
Define the role of transcription factors
Assess transfection efficiency
Convenient one-step processing: No washes required -- simply mix, incubate and read
Strong signal for remarkably accurate and thorough firefly luciferase reading
Enhanced stability: All our luciferase-based reagents are designed for extended storage at 2-8°C
No DTT: Unlike most luciferase detection reagents, ours do not contain DTT, eliminating toxic hazards and the need for hood work
Amenable to high throughput and ultra-high throughput environments, using 384- or 1536-well microplate formats
Excellent Z' Values: All systems offer robust, sensitive performance, with high signal-to-background ratios and excellent Z' values

產品與服務 (43)
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產品與服務 (43)

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Molecular Biology (9)

檢測點（200-50,000）

1000 (3)
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10000 (1)

自動化兼容

Yes (6)

濃度

1 mCi/mL (18)
11 mCi/mL (5)
0.1 mCi/mL (4)
0.05 mCi/mL (2)
0.55 mCi/mL (2)
10.25 mCi/mL (1)
31.5 mCi/mL (1)
43.3 mCi/mL (1)
5 mCi/mL (1)
10.2 mCi/mL (1)
0.02 mCi/mL (1)

檢測方法

Luminescence (6)
Radiometric (37)

實驗類型

In vitro (6)

格式

Microplates (6)

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Specifically Labeled (32)
Uniformly Labeled (3)

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溫度-最高

溫度-最低

產品品牌名稱

britelite (1)
EasyTag (1)
NEN Radiochemicals (32)
neolite (1)
sensilite (2)
steadylite plus (1)
twinlite (2)

放射性核素

³H (21)
³⁵S (9)
¹⁴C (7)

放射性同位素

C-14 (7)
H-3 (21)
S-35 (9)

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1-25 的 43 產品與服務

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Passive Lysis Solution (PLS) 1X, 100 mL

6016802

The Passive Lysis Solution (PLS) is a mammalian cell lysis solution intended to be used to prepare cell lysates for measurement of luciferase activity with the twinlite and sensilite reporter gene assay systems. It is provided in the twinlite and Sensilite kits, but if additional volume of the lysis solution is required, it is also available as a separate item, 100 mL volume per bottle.
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sensilite Enhanced Flash Luminescence (Firefly Luciferase) Reporter Gene Assay System

6066726 , 6066729

sensilite is an Enhanced Flash Firefly luciferase detection assay system designed to detect and quantitate Firefly luciferase from cultured cells with optimum sensitivity. The assay requires the use of a luminometer equipped with a dispenser as the signal has to be read shortly after dispensing.
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twinlite Firefly and Renilla Luciferase Reporter Gene Assay System

6066706 , 6066709

twinlite is a dual luciferase reporter gene assay system designed to detect and quantitate both Firefly and Renilla luciferase sequentially from cultured cells. It requires the use of a luminometer equipped with two dispensers as the signal has to be read shortly after dispensing.
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Spermidine Trihydrochloride [Terminal Methylenes-³H(N)]-

NET522001MC

Spermidine, labeled on terminal methylenes with tritium. ³H-spermidine is commonly used in spermidine transport assays.
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Serine, L-[³H(G)]

NET248250UC , NET248001MC

L-serine, labeled with tritium. ³H-serine is commonly used in serine metabolism, phospholipid labeling, and protein synthesis studies.
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Adenosyl-L-methionine, S-[methyl-³H] (SAM[³H]), 12-18 Ci/mmol

NET155V250UC , NET155V001MC

Tritiated SAM is commonly used in epigenetic methylation assays.
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Proline, L-[2,3,4,5-³H]

NET483001MC

L-proline, labeled with tritium at C2, C3, C4, C5. ³H-L-proline is commonly used in proline metabolism, amino acid uptake and release, and protein synthesis studies.
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Phenylalanine, L-[2,3,4,5,6-³H]-

NET1122001MC

L-phenylalanine, tritiated. ³H-L-phenylalanine is commonly used in protein synthesis studies and protein labeling.
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Methylaminoisobutyric Acid, α-[1-14C]-

NEC671250UC

α-Methylaminoisobutyric acid (MeAIB), labeled with C-14 at C1. ¹⁴C-MeAIB is a non-metabolizable tracer commonly used in amino acid transporter influx and placental transport studies.
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Methionine, L-[³⁵S]-, Specific Activity:>1000Ci (37.0TBq)/mMole, 10mM BME

NEG009T001MC , NEG009T005MC

Methionine amino acid labeled with ³⁵S. This formulation contains β-mercaptoethanol, and was developed for in vitro translation.
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Methionine, Cell Labeling Grade, L-[35S]-,>600 Ci (22.2 TBq) mmol, 50 mM Tricine, 10 mM BME

NEG009L005MC , NEG009L010MC

Methionine amino acid labeled with ³⁵S. This formulation contains β-mercaptoethanol.
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L-[³⁵S]-Methionine, Specific Activity:>800Ci (29.6TBq)/mMole, 50mM Tricine, 10mM BME

NEG009H001MC , NEG009H005MC

Methionine amino acid labeled with ³⁵S. This formulation contains β-mercaptoethanol.
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L-[Methyl-³H]-Methionine

NET061X250UC , NET061X001MC , NET061X005MC

L-methionine, labeled with tritium on the methyl group. ³H-L-methionine is commonly used in methionine metabolism and amino acid uptake studies.
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L-[³⁵S]-Methionine, High radioactive concentration, 50mM Tricine, 10mM BME

NEG009C005MC , NEG009C010MC

Methionine amino acid labeled with ³⁵S. This formulation is provided at high radioactive concentration, and contains β-mercaptoethanol.
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Methionine, L-[³⁵S], stabilized

NEG009A500UC , NEG009A001MC , NEG009A005MC

Methionine amino acid labeled with ³⁵S. This formulation is Tricine-stabilized, and contains β-mercaptoethanol.
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Lysine, L-[¹⁴C(U)]

NEC280E050UC , NEC280E250UC

L-lysine, uniformly labeled with C-14. ¹⁴C-L-lysine is commonly used in histone labeling of chromatin, amino acid uptake, and protein synthesis studies.
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Leucine, L-[4,5-³H]

NET1166001MC , NET1166005MC

Leucine (abbreviated as Leu or L) is an essential amino acid is used in metabolism studies.
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L-[4,5-³H(N)]-Leucine, Specific Activity 40-60Ci (1.48-2.22TBq)/mmol

NET135H005MC

L-leucine, labeled with tritium at C4, C5. ³H-L-leucine is commonly used in leucine metabolism and protein synthesis studies.
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L-[3,4,5-3H(N)]-Leucine, Aqueous Sol, Specific Activity: 100 to 150 Ci/mmol (3.7 to 5.56 TBq/mmol)

NET460A001MC , NET460A005MC

L-leucine, nominally labeled with tritium at C3, C4, C5. ³H-L-leucine is commonly used in leucine metabolism and protein synthesis studies.
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L-[3,4,5-3H(N)]-Leucine, EtOH Sol, Specific Activity: 100 to 150 Ci/mmol (3.7 to 5.56 TBq/mmol)

NET460250UC , NET460001MC

L-leucine, nominally labeled with tritium at C3, C4, C5. ³H-L-leucine is commonly used in leucine metabolism and protein synthesis studies.
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Leucine, L-[¹⁴C(U)]-, Specific Activity>300mCi (11.1GBq)/mmol

NEC279E050UC , NEC279E001MC

L-leucine, uniformly labeled with C-14. ¹⁴C-L-leucine is commonly used in protein labeling, amino acid uptake, and leucine metabolism studies.
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Isoleucine, L-[U-¹⁴C]

NEC278E050UC

L-isoleucine, uniformly labeled with C-14. ¹⁴C-L-isoleucine is commonly used in isoleucine metabolism studies.
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Glycine, [2-³H]

NET004001MC

Glycine, labeled with tritium at C2. ³H-glycine is commonly used in NMDA receptor binding, glycine uptake, and protein synthesis studies.
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[Glycine-2-³H]-Glutathione

NET282050UC , NET282250UC

Glutathione, labeled on the glycine group with tritium. ³H-glutathione is commonly used in binding and transport assays.
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Glutamine, L-[¹⁴C(U)]-

NEC451050UC

L-glutamine, uniformly labeled with C-14. ¹⁴C-L-glutamine is commonly used in glutamine transport studies.