Alpha Citations
- General
- AlphaLISA immunoassays for biomarker detection
- Biotherapeutics
- Protein-protein interactions
- DNA:protein or RNA:protein interactions
- Lipid:protein Interactions
- Nuclear receptor assays
- GPCRs
- Kinases
- Epigenetics and post-translational modifications
- Proteases and other enzymatic assays
- Antibody screening and detection
- cAMP assays
- cGMP assays
- Working with cell extracts and supernatants
- SNP detection assays
- GTPase assays
- Phosphodiesterase assays
On this page we provide selected citations for each application. To search for a more complete list of citations referencing the use of our products, please visit the 爱游戏平台注册登录 Life Sciences Citations Library.
General
Journal articles
Bielefeld-Sevigny, M. AlphaLISA Immunoassay Platform - the "No-Wash" High-Throughput Alternative to ELISA. Assay Drug Dev Technol 7, 90-92 (2009). Link
爱游戏平台注册登录 technical publications
- Bosse, R. et al. Principles of AlphaScreen. 爱游戏平台注册登录 Application Note (2001). Link
- Mai, E. et al. Comparison of ELISA and Alphascreen Assay Technologies for Measurement of Protein Expression Levels. 爱游戏平台注册登录 Application Note (2002). Link
AlphaLISA immunoassays for biomarker detection
Journal articles
- Huertas A, Tu L, Gambaryan N, Girerd B, Perros F, Montani D, Fabre D, Fadel E, Eddahibi S, Cohen-Kaminsky S, Guignabert C, Humbert M. Leptin and regulatory T lymphocytes in idiopathic pulmonary arterial hypertension. Eur Respir J 2012 Feb. Link
- Freund A, Patil CK, Campisi J. p38MAPK is a novel DNA damage response-independent regulator of the senescence-associated secretory phenotype. EMBO J. 2011 Apr;30(8):1536–1548. Link
- Cauchon, E. et al. Development of a Homogeneous Immunoassay for the Detection of Angiotensin I in Plasma Using AlphaLISA Acceptor Beads Technology. Anal Biochem 388, 134-139 (2009) Link
- Lai, M.T. et al. Antiviral activity of MK-4965, A Novel Nonnucleoside Reverse Transcriptase Inhibitor. Antimicrob Agents Chemother 53, 2424-2431 (2009). Link
- Majercak, J. et al. LRRTM3 Promotes Processing of Amyloid-Precursor Protein by BACE1 and is a Positional Candidate Gene for Late-Onset Alzheimer's Disease. Proc Natl Acad Sci USA 103, 17967-17972 (2006). Link
- Petersen, S.B. et al. Comparison of a luminescent oxygen channeling immunoassay and an ELISA for detecting insulin aspart in human serum. J Pharm Biomed Anal 51, 217-224 (2010). Link
- Poulsen, F. and Jensen, K.B. A Luminescent Oxygen Channeling Immunoassay for the Determination of Insulin in Human Plasma. J Biomol Screen 12, 240-247 (2007). Link
- Szekeres, P.G. et al. Development of Homogeneous 384-well High-Throughput Screening Assays for Abeta1-40 and Abeta1-42 using AlphaScreen technology. J Biomol Screen 13, 101-111 (2008). Link
爱游戏平台注册登录 technical publications
- Arcand, M. et al. Bacteriophage-Expressed Soluble Substrates for Protein Kinases. 爱游戏平台注册登录 Poster (2008). Link
- Bielefeld-Sévigny, M. et al. AlphaLISA: THE Fast and Easy Non-Wash Alternative to ELISA. 爱游戏平台注册登录 Poster (2007). Link
- Boissoneault, M. et al. Rapid, homogeneous and robust HIV-p24 detection assay using the AlphaLISA™ platform. 爱游戏平台注册登录 Poster. Link
- Brazzill, D. and Kealy, M. Biomarker Screening: Automated Sample Preparation for AlphaLISA Cytokine Assays using JANUS AlphaLISA Workstation. 爱游戏平台注册登录 Poster (2009). Link
- Brazzill, D. and Reznik, G. Automation of AlphaLISA Immunodetection Assays: JANUS Family of Automated Liquid Handling Workstations. 爱游戏平台注册登录 Poster. Link
- Brazzill, D. et al. Automation of AlphaLISA Insulin and VEGF Immunoassays on JANUS Liquid Handling Workstation. 爱游戏平台注册登录 Poster (2008). Link
- Brechler, V. et al. Development of New AlphaLISA No-Wash Immunoassay Kits for Sensitive, Rapid and Efficient Quantification of Cytokines. 爱游戏平台注册登录 Poster (2008). Link
- Cosentino, G. et al. AlphaLISA Assays are Homogeneous Sensitive Immunoassays for Detection of Analytesin a Variety of Biological Matrices. 爱游戏平台注册登录 Poster (2011). Link
- Dahan, S. et al. Automation and Miniaturization of Immunoassays for Drug Discovery: AlphaLISA™, a Sensitive No-Wash Assay. 爱游戏平台注册登录 Poster (2008). Link
- Howland, S. et al. Development of Customized Immunoassays using AlphaLISA. 爱游戏平台注册登录 Poster (2008). Link
- Hurt, S. et al. AlphaLISA Automation: Use of the JANUS Automated Workstation to automate AlphaLISA assays. 爱游戏平台注册登录 Application Note. Link
- Mai, E. et al. Comparison of ELISA and Alphascreen Assay Technologies for Measurement of Protein Expression Levels. 爱游戏平台注册登录 Application Note (2002). Link
- Parent, S. et al. Development of 8 New No-Wash Immunoassay Kits for the Detection of VEGF, Amyloid ß1-40, Amyloid ß1-42, Insulin, Human IgG, EPO, HIV p24 and TNFα Using 爱游戏平台注册登录 AlphaLISA. 爱游戏平台注册登录 Poster (2008). Link
- Prasad, A., Price, A., et al. A Comparison of AlphaLISA Bead-Based Luminescence and Electrochemiluminescence Immunoassay Technologies for Detection of Human EPO, Amyloid Beta 42 and VEGF in Complex Sample Matrices. Application Note by 爱游戏平台注册登录 and University of Kansas (2010). Link
- Prasad, A. et al. A Comparison of a Homogeneous Bead-Based Amplified Luminescence Technology AlphaLISA and a Colorimetric ELISA for Detection of Human Matrix Metalloproteinase 9 in Complex Sample Matrices. 爱游戏平台注册登录 Application Note (2010). Link
- Prasad, A. et al. Detection of sAPPα in Human CSF: A Comparison Between AlphaLISA and Standard Sandwich ELISA. 爱游戏平台注册登录 Application Note (2010) Link
- Rodenbrock, A. et al. Analyte Depletion of Serum for Quantitative AlphaLISA Assays. 爱游戏平台注册登录 Application Note (2008). Link
- Rodriguez-Suarez, R. et al. All-In-One-Well AlphaLISA Assays for Direct Biomarker Quantification in Cell Culture. 爱游戏平台注册登录 Poster (2009). Link
- Rouleau, N. et al. Highly Sensitive Detection of the Interaction Occurring Between Phage Displayed Peptides and their Target Using AlphaScreen. 爱游戏平台注册登录 Poster. Link
- Talvitie, M. Evaluating a TNF-α immunoassay using EnSpire AlphaPLUS: ELISA and AlphaLISA technologies. 爱游戏平台注册登录 Application Note (2009). Link
Biotherapeutics
- Houde D, Berkowitz SA. Conformational comparability of factor IX-Fc fusion protein, factor IX, and purified Fc fragment in the absence and presence of calcium. Journal of Pharmaceutical Sciences 2012 Jan. Link
- Goetze AM, Liu YD, Arroll T, Chu L, Flynn GC. Rates and Impact of Human Antibody Glycation In Vivo. Glycobiology 2011 Sep. Link
- Nakamura S, Morimoto N, Tsuruma K, Izuta H, Yasuda Y, Kato N, Ikeda T, Shimazawa M, Hara H. Tissue kallikrein inhibits retinal neovascularization via the cleavage of vascular endothelial growth factor-165. Arterioscler. Thromb. Vasc. Biol. 2011 May;31(5):1041–1048. Link
- Mazella J, Pétrault O, Lucas G, Deval E, Béraud-Dufour S, Gandin C, El-Yacoubi M, Widmann C, Guyon A, Chevet E, Taouji S, Conductier G, Corinus A, Coppola T, Gobbi G, Nahon J-L, Heurteaux C, Borsotto M. Spadin, a sortilin-derived peptide, targeting rodent TREK-1 channels: a new concept in the antidepressant drug design. PLoS Biol 2010;8(4):e1000355. Link
- Saitoh R, Ohtomo T, Yamada Y, Kamada N, Nezu J-I, Kimura N, Funahashi S-I, Furugaki K, Yoshino T, Kawase Y, Kato A, Ueda O, Jishage K-I, Suzuki M, Fukuda R, Arai M, Iwanari H, Takahashi K, Sakihama T, Ohizumi I, Kodama T, Tsuchiya M, Hamakubo T. Viral envelope protein gp64 transgenic mouse facilitates the generation of monoclonal antibodies against exogenous membrane proteins displayed on baculovirus. J. Immunol. Methods 2007 Apr;322(1-2):104–117. Link
- Lazar GA, Dang W, Karki S, Vafa O, Peng JS, Hyun L, Chan C, Chung HS, Eivazi A, Yoder SC, Vielmetter J, Carmichael DF, Hayes RJ, Dahiyat BI. Engineered antibody Fc variants with enhanced effector function. Proc. Natl. Acad. Sci. U.S.A 2006 Mar;103(11):4005–10. Link
- Miller MD, Geleziunas R, Bianchi E, Lennard S, Hrin R, Zhang H, Lu M, An Z, Ingallinella P, Finotto M, Mattu M, Finnefrock AC, Bramhill D, Cook J, Eckert DM, Hampton R, Patel M, Jarantow S, Joyce J, Ciliberto G, Cortese R, Lu P, Strohl W, Schleif W, McElhaugh M, Lane S, Lloyd C, Lowe D, Osbourn J, Vaughan T, Emini E, Barbato G, Kim PS, Hazuda DJ, Shiver JW, Pessi A. A human monoclonal antibody neutralizes diverse HIV-1 isolates by binding a critical gp41 epitope. Proc. Natl. Acad. Sci. U.S.A 2005 Oct;102(41):14759–64. Link
Protein-protein interactions
Journal articles
Cell-based protein-protein interactions
- Bovijn C, Ulrichts P, De Smet A-S, Catteeuw D, Beyaert R, Tavernier J, Peelman F. Identification of Interaction Sites for Dimerization and Adapter Recruitment in Toll/Interleukin-1 Receptor (TIR) Domain of Toll-like Receptor 4. J. Biol. Chem. 2012 Feb;287(6):4088–4098. Link
- Tang X, Seyb KI, Huang M, Schuman ER, Shi P, Zhu H, Glicksman MA. A High-Throughput Screening Method for Small-Molecule Inhibitors of the Aberrant Mutant SOD1 and Dynein Complex Interaction. J Biomol Screen 2012 Mar;17(3):314–326. Link
- Vosjan MJWD, Vercammen J, Kolkman JA, Stigter-van Walsum M, Revets H, van Dongen GAMS. Nanobodies targeting the hepatocyte growth factor: potential new drugs for molecular cancer therapy. Mol. Cancer Ther 2012 Feb. Link
- Becker MN, Todd TM, Moyer RW. An Amsacta moorei entomopoxvirus ortholog of the poly(A) polymerase small subunit exhibits methyltransferase activity and is non-essential for virus growth. Virology 2008 Jun;375(2):624–636. Link
- Mohamed MR, Rahman MM, Lanchbury JS, Shattuck D, Neff C, Dufford M, van Buuren N, Fagan K, Barry M, Smith S, Damon I, McFadden G. Proteomic screening of variola virus reveals a unique NF-kappaB inhibitor that is highly conserved among pathogenic orthopoxviruses. Proc. Natl. Acad. Sci. U.S.A 2009 Jun;106(22):9045–9050. Link
- Rahman MM, Mohamed MR, Kim M, Smallwood S, McFadden G. Co-regulation of NF-kappaB and inflammasome-mediated inflammatory responses by myxoma virus pyrin domain-containing protein m013. PLoS Pathog 2009 Oct;5(10):e1000635. Link
- Lavens D, Peelman F, Van der Heyden J, Uyttendaele I, Catteeuw D, Verhee A, Van Schoubroeck B, Kurth J, Hallenberger S, Clayton R, Tavernier J. Definition of the interacting interfaces of Apobec3G and HIV-1 Vif using MAPPIT mutagenesis analysis. Nucleic Acids Res 2009 Dec. Link
- Werden SJ, McFadden G. Pharmacological manipulation of the Akt signaling pathway regulates myxoma virus replication and tropism in human cancer cells. J Virol 2010 Jan;84(7):3287–3302. Link
- Waller H, Chatterji U, Gallay P, Parkinson T, Targett-Adams P. The use of AlphaLISA technology to detect interaction between hepatitis C virus-encoded NS5A and cyclophilin A. J Virol Methods 2010 Feb. Link
Biochemical protein-protein interactions
- Cavalluzzo C, Voet A, Christ F, Singh BK, Sharma A, Debyser Z, Maeyer MD, Eycken EV der. De novo design of small molecule inhibitors targeting the LEDGF/p75-HIV integrase interaction. RSC Advances 2012;2(3):974.
- Demeulemeester J, Tintori C, Botta M, Debyser Z, Christ F. Development of an AlphaScreen-Based HIV-1 Integrase Dimerization Assay for Discovery of Novel Allosteric Inhibitors. J Biomol Screen 2012 Feb. Link
- Houde D, Berkowitz SA. Conformational comparability of factor IX-Fc fusion protein, factor IX, and purified Fc fragment in the absence and presence of calcium. Journal of Pharmaceutical Sciences 2012 Jan. Link
- Madono M, Sawasaki T, Morishita R, Endo Y. Wheat germ cell-free protein production system for post-genomic research. N Biotechnol 2011 Apr;28(3):211–217. Link
- Goetze AM, Liu YD, Arroll T, Chu L, Flynn GC. Rates and Impact of Human Antibody Glycation In Vivo. Glycobiology 2011 Sep. Link
- Garcia BL, Summers BJ, Lin Z, Ramyar KX, Ricklin D, Kamath DV, Fu Z-Q, Lambris JD, Geisbrecht BV. Diversity in the C3B contact residues and tertiary structures of the staphylococcal complement inhibitor (SCIN) protein family. J Biol Chem 2011 Nov. Link
- Cassel JA, Blass BE, Reitz AB, Pawlyk AC. Development of a novel nonradiometric assay for nucleic acid binding to TDP-43 suitable for high-throughput screening using AlphaScreen technology. J Biomol Screen 2010 Oct;15(9):1099–1106. Link
- Kota S, Coito C, Mousseau G, Lavergne J-P, Strosberg AD. Peptide inhibitors of hepatitis C virus core oligomerization and virus production. J. Gen. Virol 2009 Jun;90(Pt 6):1319–1328. Link
- Lazar GA, Dang W, Karki S, Vafa O, Peng JS, Hyun L, Chan C, Chung HS, Eivazi A, Yoder SC, Vielmetter J, Carmichael DF, Hayes RJ, Dahiyat BI. Engineered antibody Fc variants with enhanced effector function. Proc. Natl. Acad. Sci. U.S.A 2006 Mar;103(11):4005–10. Link
爱游戏平台注册登录 technical publications
- Barbeau, A., Banks, P. & Wenham, D. Screening for Inhibitors to TNFalpha/TNFR1 Binding Using AlphaScreen Technology. 爱游戏平台注册登录 Application Note. Link
- Illy, C. et al. Homogeneous Detection and Measurement of Micromolar Affinity Interactions Using AlphaScreen. 爱游戏平台注册登录 Application Note (with Merck) (2002). Link
- Rouleau, N. et al. Highly Sensitive Detection of the Interaction Occuring Between Phage Displayed Peptides and their Target using AlphaScreen. Application Note by 爱游戏平台注册登录 and DYAX (2002). Link
- Gβγ-GIRK1 Interaction Assay. 爱游戏平台注册登录 Technical Note (2003). Link
DNA:protein or RNA:protein interactions
Journal articles
- McNeely M, Hendrix J, Busschots K, Boons E, Deleersnijder A, Gerard M, Christ F, Debyser Z. In vitro DNA tethering of HIV-1 integrase by the transcriptional coactivator LEDGF/p75. J. Mol. Biol 2011 Jul;410(5):811–830. Link
- Stutz A, Bertheloot D, Latz E. Innate immune receptors for nucleic acids. Methods Mol. Biol 2011;748:69–82. Link
- Dausse E, Taouji S, Evadé L, Di Primo C, Chevet E, Toulmé J-J. HAPIscreen, a method for high-throughput aptamer identification. J Nanobiotechnology 2011;9:25. Link
- Mills NL, Shelat AA, Guy RK. Assay Optimization and Screening of RNA-Protein Interactions by AlphaScreen. J Biomol Screen 2007 Oct;12(7):946–55. Link
爱游戏平台注册登录
technical publications
- AlphaScreen ERα Binding Assay. 爱游戏平台注册登录 Technical Note (2000). Link
Lipid:protein Interactions
Journal articles
- Malapaka RRV, Khoo SK, Zhang J, Choi JH, Zhou XE, Xu Y, Gong Y, Li J, Yong E-LE, Chalmers MJ, Chang L, Resau JH, Griffin PR, Chen YE, Xu HE. Identification and mechanism of a ten carbon fatty acid as a modulating ligand of peroxisome proliferator activated receptors. J Biol Chem 2011 Oct. Link
- Jerga A, Miller DJ, White SW, Rock CO. Molecular determinants for interfacial binding and conformational change in a soluble diacylglycerol kinase. J. Biol. Chem 2009 Mar;284(11):7246–7254. Link
- Rzepecki PW, Prestwich GD. Synthesis of hybrid lipid probes: derivatives of phosphatidylethanolamine-extended phosphatidylinositol 4,5-bisphosphate (Pea-PIP(2)). J. Org. Chem 2002 Aug;67(16):5454–5460. Link
Nuclear receptor assays
Journal articles
- Malapaka RRV, Khoo SK, Zhang J, Choi JH, Zhou XE, Xu Y, Gong Y, Li J, Yong E-LE, Chalmers MJ, Chang L, Resau JH, Griffin PR, Chen YE, Xu HE. Identification and mechanism of a ten carbon fatty acid as a modulating ligand of peroxisome proliferator activated receptors. J Biol Chem 2011 Oct. Link
- Istrate MA, Spicer TP, Wang Y, Bernard JA, Helvering LM, Bocchinfuso WP, Richardson TI, Zink R, Kumar N, Montrose-Rafizadeh C, Dodge J, Hodder P, Griffin PR. Development of an HTS-Compatible Assay for Discovery of ROR{alpha} Modulators Using AlphaScreen(R) Technology. J Biomol Screen 2011 Feb;16(2):183–191. Link
- Zhou XE, Suino-Powell KM, Xu Y, Chan C-W, Tanabe O, Kruse SW, Reynolds R, Engel JD, Xu HE. The Orphan Nuclear Receptor TR4 Is a Vitamin A-activated Nuclear Receptor. J. Biol. Chem 2011 Jan;286(4):2877–2885. Link
- Partch, C.L. & Card, P.B., Amezcua, C.A. & Gardner, K.H. Molecular Basis of Coiled Coil Coactivator Recruitment by the Aryl Hydrocarbon Receptor Nuclear Translocator (ARNT). J Biol Chem 284, 15184-15192 (2009). Link
- Wagstaff, K.M. & Jans, D.A. Intramolecular Masking of Nuclear Localization Signals: Analysis of Importin Binding Using a novel AlphaScreen-based method. Anal Biochem 348, 49-56 (2006). Link
- Wang, W. et al. The Crystal Structures of Human Steroidogenic Factor-1 and Liver Receptor Homologue-1. Proc Natl Acad Sci USA 102, 7505-7510 (2005). Link
- Bramlett, K.S. et al. A Natural Product Ligand of the Oxysterol Receptor, Liver X Receptor. J Pharmacol Exp Ther 307, 291-296 (2003). Link
- Rouleau, N. et al. Development of a Versatile Platform for Nuclear Receptor Screening Using AlphaScreen. J Biomol Screen 8, 191-197 (2003). Link
- Xu H.E. et al. Structural Basis for Antagonist-Mediated Recruitment of Nuclear Co-Repressors by PPARalpha. Nature 415, 813-817 (2002). Link
- Glickman, J.F. et al. A Comparison of ALPHAScreen, TR-FRET, and TRF as Assay Methods for FXR Nuclear Receptors. J Biomol Screen 7, 3-10 (2002). Link
爱游戏平台注册登录
technical publications
- Rouleau, N., Turcotte, S., Roby, P. & Bosse, R. Development of a Homogeneous Luminescent HTS Platform for Detection of Nuclear Receptor Modulators Using the AlphaScreen Technology. 爱游戏平台注册登录 Poster. Link
- AlphaScreen ERα Binding Assay. 爱游戏平台注册登录 Technical Note (2000). Link
GPCRs
Journal articles
- Bartho JD, Ly K, Hay DL. Measurement of cAMP in an Undergraduate Teaching Laboratory, Using ALPHAscreen Technology. Sci. Signal. 2012 Feb;5(211):tr1. Link
- van Koppen CJ, de Gooyer ME, Karstens W-J, Plate R, Conti PGM, van Achterberg TAE, van Amstel MGA, Brands JHGM, Wat J, Berg RJW, Lane JRD, Miltenburg AMM, Timmers CM. Mechanism of Action of a Nanomolar Potent, Allosteric Antagonist of the Thyroid-Stimulating Hormone Receptor. Br. J. Pharmacol. 2011 Oct. Link
- Uitdehaag JCM, Sünnen CM, van Doornmalen AM, de Rouw N, Oubrie A, Azevedo R, Ziebell M, Nickbarg E, Karstens W-J, Ruygrok S. Multidimensional Profiling of CSF1R Screening Hits and Inhibitors: Assessing Cellular Activity, Target Residence Time, and Selectivity in a Higher Throughput Way. J Biomol Screen 2011 Aug. Link
- Hay DL. Measurement of Phosphorylated Extracellular Signal-Regulated Kinase 1 and 2 in an Undergraduate Teaching Laboratory with ALPHAscreen Technology. Sci. Signal. 2009 Mar;2(62):tr1. Link
- Pioszak, A.A., Parker, N.R., Suino-Powell, K. & Xu, H.E. Molecular Recognition of Corticotropin-Releasing Factor by its G-Protein-Coupled Receptor CRFR1. J Biol Chem 283, 32900-32912 (2008). Link
- Pioszak, A.A. & Xu, H.E. Molecular Recognition of Parathyroid Hormone by Its G Protein-Coupled Receptor. Proc Natl Acad Sci USA 105, 5034-5039 (2008). Link
- Siehler, S. Cell-Based Assays in GPCR Drug Discovery. Biotechnol J 3, 471-483 (2008). Link
Kinases
Journal articles
Biochemical
- Arai R, Tsuda M, Watanabe T, Ose T, Obuse C, Maenaka K, Minami A, Ohba Y. Simultaneous inhibition of Src and Aurora kinases by SU6656 induces therapeutic synergy in human synovial sarcoma growth, invasion and angiogenesis in vivo. European Journal of Cancer (Oxford, England: 1990) 2012 Jan. Link
- Gao Z-G, Verzijl D, Zweemer A, Ye K, Göblyös A, Ijzerman AP, Jacobson KA. Functionally biased modulation of A(3) adenosine receptor agonist efficacy and potency by imidazoquinolinamine allosteric enhancers. Biochem. Pharmacol. 2011 Sep;82(6):658–668. Link
- Babij C, Zhang Y, Kurzeja RJ, Munzli A, Shehabeldin A, Fernando M, Quon K, Kassner PD, Ruefli-Brasse AA, Watson VJ, Fajardo F, Jackson A, Zondlo J, Sun Y, Ellison AR, Plewa CA, San MT, Robinson J, McCarter J, Schwandner R, Judd T, Carnahan J, Dussault I. STK33 Kinase Activity Is Nonessential in KRAS-Dependent Cancer Cells. Cancer Res 2011 Sep;71(17):5818–5826. Link
- Peterson EA, Andrews PS, Be X, Boezio AA, Bush TL, Cheng AC, Coats JR, Colletti AE, Copeland KW, DuPont M, Graceffa R, Grubinska B, Harmange J-C, Kim JL, Mullady EL, Olivieri P, Schenkel LB, Stanton MK, Teffera Y, Whittington DA, Cai T, La DS. Discovery of triazine-benzimidazoles as selective inhibitors of mTOR. Bioorg. Med. Chem. Lett 2011 Apr;21(7):2064–2070. Link
- Li, Y. et al. Homogeneous Assays for Adenosine 5'-Monophosphate-Activated Protein Kinase. Anal Biochem 321, 151-156 (2003). Link
- Von Leoprechting, A. et al. Miniaturization and Validation of a High-Throughput Serine Kinase Assay Using the AlphaScreen Platform. J Biomol Screen 9, 719-725 (2004). Link
- Warner, G. et al. AlphaScreen Kinase HTS Platforms. Curr Med Chem 11, 721-730 (2004). Link
Cellular
- Allen JV, Bardelle C, Blades K, Buttar D, Chapman L, Colclough N, Dossetter AG, Garner AP, Girdwood A, Lambert C, Leach AG, Law B, Major J, Plant H, Slater AM. The discovery of benzanilides as c-Met receptor tyrosine kinase inhibitors by a directed screening approach. Bioorg. Med. Chem. Lett 2011 Sep;21(18):5224–5229. Link
- Babij C, Zhang Y, Kurzeja RJ, Munzli A, Shehabeldin A, Fernando M, Quon K, Kassner PD, Ruefli-Brasse AA, Watson VJ, Fajardo F, Jackson A, Zondlo J, Sun Y, Ellison AR, Plewa CA, San MT, Robinson J, McCarter J, Schwandner R, Judd T, Carnahan J, Dussault I. STK33 kinase activity is nonessential in KRAS-dependent cancer cells. Cancer Res 2011 Sep;71(17):5818–5826. Link
- Drew AE, Al-Assaad S, Yu V, Andrews P, Merkel P, Szilvassy S, Emkey R, Lewis R, Brake RL. Comparison of 2 Cell-Based Phosphoprotein Assays to Support Screening and Development of an ALK Inhibitor. J Biomol Screen 2011 Feb;16(2):164–173. Link
- Boldyreff, B. et al. Expression and Purification of PI3 Kinase Alpha and Development of an ATP Depletion and an Alphascreen PI3 Kinase Activity Assay. J Biomol Screen 13, 1035-1040 (2008). Link
- Guenat, S. et al. Homogeneous and Nonradioactive High-Throughput Screening Platform for the Characterization of Kinase Inhibitors in Cell Lysates. J Biomol Screen 11, 1015-1026 (2006). Link
- Hay, D.L. Measurement of Phosphorylated Extracellular Signal-Regulated Kinase 1 and 2 in an Undergraduate Teaching Laboratory with ALPHAscreen Technology Sci Signal 2, tr1 (2009). Link
- Smotrov, N. et al. Development of a Cell-Based Assay for Measurement of c-Met Phosphorylation Using AlphaScreen Technology and High-Content Imaging Analysis. J Biomol Screen 14, 404-411 (2009). Link
爱游戏平台注册登录 technical publications
- Arcand, M. et al. Bacteriophage-Expressed Soluble Substrates for Protein Kinases. 爱游戏平台注册登录 Poster (2008). Link
- Arcand, M. et al. Single-well simultaneous measurement of MAP2K MEK1 activity and interaction with the MAP kinase ERK2 using AlphaScreen and AlphaLISA platforms. 爱游戏平台注册登录 Poster (2009). Link
- Bedard, J. et al. JNK3 activity assay using AlphaScreen. 爱游戏平台注册登录 Application Note (2005). Link
- Bedard, J. et al. Homogeneous and High Throughput Characterization of DNA Damage Based on Cell-Based Detection of p53 Phosphorylation. Poster by 爱游戏平台注册登录 , TGR BioSciences, Cell Signaling Technologies, and the National Institutes of Health. Link
- Bielefeld-Sévigny, M. et al. Homogeneous and non-radioactive Cellular Assay Platforms for the Characterization of Kinase Inhibitors. Poster by 爱游戏平台注册登录 and University of Lausanne. Link
- Bosse, R. et al. Development of a Homogenous and Label-less AlphaScreen® Based Platform to Measure MEK2 Activity. Poster by 爱游戏平台注册登录 and UCB Celltech (2006). Link
- Bouchard, N. et al. Ultra-Sensitive Detection of Akt Kinase Activity Using AlphaScreen. Poster by 爱游戏平台注册登录 and Cell Signaling Technology. Link
- Geron, I. et al. Homogeneous Antibody-Free detection of Tyrosine and Serine/Threonine Kinase Activity. 爱游戏平台注册登录 Poster (2005). Link
- St. Pierre, J. et al. AlphaScreen PI 3-kinase Assay: A Homogeneous, High-Throughput Assay for Screening Modulators of PI 3-Kinase Activity. Poster by 爱游戏平台注册登录 and Echelon Biosciences Inc. Link
- True, D. et al. Comparison of Kinase Assay Technologies for High Throughput Screening. 爱游戏平台注册登录 Poster (2002). Link
- Warner, G. et al. Development of a Homogeneous p38 Kinase Assay using AlphaScreen™ Technology. Application Note by 爱游戏平台注册登录 and Cell Signaling Technology (2002). Link
- AlphaScreen MAP Kinase Assay. 爱游戏平台注册登录 Application Note (2001). Link
- P-Tyr-100 Insulin Receptor Tyrosine Kinase Assay. 爱游戏平台注册登录 Application Note (2003). Link
Epigenetics and post-translational modifications
Journal articles
- Herold, J. M. et al. Structure–activity relationships of methyl-lysine reader antagonists. MedChemComm3, 45 (2012).
- Luo M. Current Chemical Biology Approaches to Interrogate Protein Methyltransferases. ACS Chemical Biology 2012 Jan. Link
- Falk H, Connor T, Yang H, Loft KJ, Alcindor JL, Nikolakopoulos G, Surjadi RN, Bentley JD, Hattarki MK, Dolezal O, Murphy JM, Monahan BJ, Peat TS, Thomas T, Baell JB, Parisot JP, Street IP. An Efficient High-Throughput Screening Method for MYST Family Acetyltransferases, a New Class of Epigenetic Drug Targets. J Biomol Screen 2011 Nov. Link
- Guo W, Qian L, Zhang J, Zhang W, Morrison A, Hayes P, Wilson S, Chen T, Zhao J. Sirt1 overexpression in neurons promotes neurite outgrowth and cell survival through inhibition of the mTOR signaling. J. Neurosci. Res 2011 Nov;89(11):1723–1736. Link
- Philpott M, Yang J, Tumber T, Fedorov O, Uttarkar S, Filippakopoulos P, Picaud S, Keates T, Felletar I, Ciulli A, Knapp S, Heightman TD. Bromodomain-peptide displacement assays for interactome mapping and inhibitor discovery. Mol Biosyst 2011 Oct;7(10):2899–2908. Link
- Hauser A-T, Bissinger E-M, Metzger E, Repenning A, Bauer U-M, Mai A, Schüle R, Jung M. Screening Assays for Epigenetic Targets Using Native Histones as Substrates. J Biomol Screen 2011 Sep. Link
- Gauthier N, Caron M, Pedro L, Arcand M, Blouin J, Labonté A, Normand C, Paquet V, Rodenbrock A, Roy M, Rouleau N, Beaudet L, Padrós J, Rodriguez-Suarez R. Development of Homogeneous Nonradioactive Methyltransferase and Demethylase Assays Targeting Histone H3 Lysine 4. J Biomol Screen 2011 Sep. Link
- Goetze AM, Liu YD, Arroll T, Chu L, Flynn GC. Rates and Impact of Human Antibody Glycation In Vivo. Glycobiology 2011 Sep. Link
- Ferguson AD, Larsen NA, Howard T, Pollard H, Green I, Grande C, Cheung T, Garcia-Arenas R, Cowen S, Wu J, Godin R, Chen H, Keen N. Structural Basis of Substrate Methylation and Inhibition of SMYD2. Structure 2011 Sep;19(9):1262–1273. Link
- Quinn, A. et al. A homogeneous method for investigation of methylation-dependent protein-protein interactions in epigenetics. Nucl Acids Res , 1-10 (2009). Link
- Salahudeen, A.A. et al. An E3 ligase possessing an iron-responsive hemerythrin domain is a regulator of iron homeostasis. Science 326, 722-726 (2009). Link
- Wigle, T.J. et al. Screening for Inhibitors of Low-Affinity Epigenetic Peptide-Protein Interactions: An AlphaScreen-Based Assay for Antagonists of Methyl-Lysine Binding Proteins. J Biomol Screen (2009). Link
- Wallace, M. et al. Dual-Site Regulation of MDM2 E3-Ubiquitin Ligase Activity. Mol Cell 23, 251-263 (2006). Link
- Kus, B. et al. A High Throughput Screen to Identify Substrates for the Ubiquitin Ligase Rsp5. J Biol Chem 280, 29470-29478 (2005). Link
Proteases and other enzymatic assays
Journal articles
- Akagi T, Shimizu K, Takahama S, Iwasaki T, Sakamaki K, Endo Y, Sawasaki T. Caspase-8 cleavage of the interleukin-21 (IL-21) receptor is a negative feedback regulator of IL-21 signaling. FEBS Lett 2011 Jun;585(12):1835–1840. Link
- Madono M, Sawasaki T, Morishita R, Endo Y. Wheat germ cell-free protein production system for post-genomic research. N Biotechnol 2011 Apr;28(3):211–217. Link
- Marchand, C. et al. Identification of Phosphotyrosine Mimetic Inhibitors of Human Tyrosyl-DNA Phosphodiesterase I by a Novel AlphaScreen High-Throughput Assay. Mol Cancer Ther 8, 240-248 (2009). Link
- Espeseth, A.S. et al. Compounds that Bind APP and Inhibit Abeta Processing in Vitro Suggest a Novel Approach to Alzheimer Disease Therapeutics. J Biol Chem 280, 17792-17797 (2005). Link
- Peppard. J. et al. Development of a High-Throughput Screening Assay for Inhibitors of Aggrecan Cleavage Using Luminescent Oxygen Channeling (AlphaScreen). J Biomol Screen 8, 149-156 (2003). Link
爱游戏平台注册登录 technical publications
- Ablenas, C. et al. Simple Protease Assays Using Whole-Protein Substrates. 爱游戏平台注册登录 Poster (2008). Link
- Ablenas, C. et al. BACE-1 and Caspase-3 AlphaLISA Protease Assays Using Whole-Protein Substrates. 爱游戏平台注册登录 Poster (2009). Link
Antibody screening and detection
Journal articles
- Vosjan MJWD, Vercammen J, Kolkman JA, Stigter-van Walsum M, Revets H, van Dongen GAMS. Nanobodies targeting the hepatocyte growth factor: potential new drugs for molecular cancer therapy. Mol. Cancer Ther 2012 Feb. Link
- Goetze AM, Liu YD, Arroll T, Chu L, Flynn GC. Rates and Impact of Human Antibody Glycation In Vivo. Glycobiology 2011 Sep. Link
- Stewart R, Thom G, Levens M, Güler-Gane G, Holgate R, Rudd PM, Webster C, Jermutus L, Lund J. A variant human IgG1-Fc mediates improved ADCC. Protein Eng. Des. Sel. 2011 Sep;24(9):671–678. Link
- Tsuge S, Mizutani Y, Matsuoka K, Sawasaki T, Endo Y, Naruishi K, Maeda H, Takashiba S, Shiogama K, Inada K-I, Tsutsumi Y. Specific in situ visualization of plasma cells producing antibodies against Porphyromonas gingivalis in gingival radicular cyst: application of the enzyme-labeled antigen method. J. Histochem. Cytochem 2011 Jul;59(7):673–689. Link
- Bembenek ME, Burkhardt A, Ma J, Li Z, Loke H-K, Wu D, Xu Q, Tayber O, Xie L, Li P, Li L. Determination of complementary antibody pairs using protein A capture with the AlphaScreen assay format. Anal. Biochem 2011 Jan;408(2):321–327. Link
- Lazar, G.A. et al. Engineered Antibody Fc Variants With Enhanced Effector Function. Proc Natl Acad Sci USA 103, 4005-4010 (2006). Link
- Miller, M.D. et al. A Human Monoclonal Antibody Neutralizes Diverse HIV-1 Isolates by Binding a Critical gp41 Epitope. Proc Natl Acad Sci USA 102, 14759-14764 (2005). Link
cAMP assays
- Bartho JD, Ly K, Hay DL. Measurement of cAMP in an Undergraduate Teaching Laboratory, Using ALPHAscreen Technology. Sci. Signal. 2012 Feb;5(211):tr1. Link
- Bailey RJ, Walker CS, Ferner AH, Loomes KM, Prijic G, Halim A, Whiting L, Phillips ARJ, Hay DL. Pharmacological characterisation of rat amylin receptors: implications for the identification of amylin receptor subtypes. Br. J. Pharmacol. 2011 Oct. Link
- Qiu Y, Wang Y, Law P-Y, Chen H-Z, Loh HH. Cholesterol regulates micro-opioid receptor-induced beta-arrestin 2 translocation to membrane lipid rafts. Mol. Pharmacol 2011 Jul;80(1):210–218. Link
- Breit, A., Lagacé, M. & Bouvier, M. Hetero-Oligomerization Between Beta2- and Beta3-Adrenergic Receptors Generates a Beta-Adrenergic Signaling Unit With Distinct Functional Properties. J Biol Chem 279, 28756-28765 (2004). Link
- Elster, L., Elling, C. & Heding, A. Bioluminescence Resonance Energy Transfer as a Screening Assay: Focus on Partial and Inverse Agonism. J Biomol Screen 12, 41-49 (2007). Link
- Moll, D. et al. Biomolecular Interaction Analysis in Functional Proteomics. J Neural Transm 113, 1015-1032 (2006). Link
- Slominski, A., et al. CRH Functions as a Growth Factor/Cytokine in the Skin. J Cell Physiol 206, 780-791 (2006). Link
爱游戏平台注册登录
technical publications
- Bosse, R., Illy, C., Beaudet, L., Wang, C. & Chelsky, D. ALPHASCREEN™ for 384- and 1536-Well Microformatted Assays. 爱游戏平台注册登录 Poster (2000). Link
- Bosse, R. et al. ALPHASCREEN™ to Measure cAMP induction with SIGNALSCREEN™ Dopamine D1 receptor membranes. 爱游戏平台注册登录 Poster (2000). Link
- Bosse, R. et al. Rapid and Ultra-Sensitive Automated Detection of cAMP and IP3 Using the Homogeneous and Non-Radioactive HTS Platform AlphaScreen. 爱游戏平台注册登录 Poster (2002). Link
- Bouchard, N., Robitaille, E. & Wenham, D. cAMP AlphaScreen Assay: A method for the pharmacological characterization and screening of Gi-coupled receptors in whole cells. 爱游戏平台注册登录 Application Note (2006). Link
- Kasila, P. & Harney, H. Comparison of cAMP Assay Technologies for High Throughput Screening. 爱游戏平台注册登录 Poster (2002). Link
- Kasila, P. et al. Cell-based assays in 1536-well formats - Homogeneous, highly sensitive cAMP detection systems. 爱游戏平台注册登录 Poster. Link
cGMP assays
Journal articles
- St Pierre, L. et al. Cloning and Characterisation of Natriuretic Peptides from the Venom Glands of Australian Elapids. Biochimie 88, 1923-1931 (2006). Link
- Tessler, R. et al. Sildenafil Acutely Reverses the Hypoxic Pulmonary Vasoconstriction Response of the Newborn Pig. Pediatric Research 64, 251-255 (2008). Link
爱游戏平台注册登录
technical publications
- Boissoneault, M. et al. cGMP Phosphodiesterase Assay. 爱游戏平台注册登录 Application Note (2006). Link
- Rouleau, N. et al. Development of a Non-Radioactive Homogeneous HTS Platform to Measure the Activity of Guanylate Cyclase. 爱游戏平台注册登录 Poster (2004). Link
- AlphaScreen Functional cGMP Assay: Sensitive, flexible and cost efficient. 爱游戏平台注册登录 Application Note (2006). Link
Working with cell extracts and supernatants
Journal articles
- Bembenek ME, Burkhardt A, Ma J, Li Z, Loke H-K, Wu D, Xu Q, Tayber O, Xie L, Li P, Li L. Determination of complementary antibody pairs using protein A capture with the AlphaScreen assay format. Anal. Biochem 2011 Jan;408(2):321–327. Link
- Tang X, Seyb KI, Huang M, Schuman ER, Shi P, Zhu H, Glicksman MA. A High-Throughput Screening Method for Small-Molecule Inhibitors of the Aberrant Mutant SOD1 and Dynein Complex Interaction. J Biomol Screen 2012 Mar;17(3):314–326. Link
- Bammens L, Chavez-Gutierrez L, Tolia A, Zwijsen A, De Strooper B. Functional and topological analysis of Pen-2, the fourth subunit of the gamma-secretase complex. J Biol Chem 2011 Feb. Link
- Waller H, Chatterji U, Gallay P, Parkinson T, Targett-Adams P. The use of AlphaLISA technology to detect interaction between hepatitis C virus-encoded NS5A and cyclophilin A. J Virol Methods 2010 Feb. Link
- Szekeres, P.G. et al. Development of Homogeneous 384-Well High-Throughput Screening Assays for Abeta1-40 and Abeta1-42 Using AlphaScreen Technology. J Biomol Screen 2008;13(2):101-11. Link
- Guenat, S. et al. Homogeneous and Nonradioactive High-Throughput Screening Platform for the Characterization of Kinase Inhibitors in Cell Lysates. J Biomol Screen 11, 1015-1026 (2006). Link
爱游戏平台注册登录
technical publications
- Prasad, A. et al. Cytokine detection in Culture Media Containing 10% FBS. Link
- Prasad, A. et al. A Comparison of a Homogeneous Bead-Based Amplified Luminescence Technology AlphaLISA and a Colorimetric ELISA for Detection of Human Matrix Metalloproteinase 9 in Complex Sample Matrices. 爱游戏平台注册登录 Poster (2009). Link
- Rodriguez-Suarez, R. et al. All-In-One-Well AlphaLISA Assays for Direct Biomarker Quantification in Cell Culture. 爱游戏平台注册登录 Poster (2009). Link
SNP detection assays
Journal articles
- Beaudet, L. et al. Homogeneous Assays for Single-Nucleotide Polymorphism Typing Using AlphaScreen. Genome Res 11, 600-608 (2001). Link
GTPase assays
Journal articles
- Niebel, B. et al. A luminescent oxygen channeling biosensor that measures small GTPase activation. Chem. Commun. (Camb.)47, 7521–7523 (2011). Link
- Caruso, M. et al. Biochemical Clustering of Monomeric GTPases of the Ras Superfamily. Mol Cell Proteomics 4, 936-944 (2005). Link
爱游戏平台注册登录 technical publications
- Caruso, M. et al. Reverse-Proteomic Analysis of Rho GTPase Regulation by RhoGAPs using AlphaScreen. Poster by McGill University, Biotechnology Research Institute, Dana-Farber Cancer Institute, and 爱游戏平台注册登录 (2003). Link
- Lassle, M. et al. High Throughput Assays for the Characterization of Monomeric GTPases of the Ras Superfamily. Poster by 爱游戏平台注册登录 , McGill University, U.Q.A.M., University of Montreal, and Dana-Farber Cancer Institute. Link
Phosphodiesterase assays
Journal articles
- Weidlich IE, Dexheimer T, Marchand C, Antony S, Pommier Y, Nicklaus MC. Inhibitors of human tyrosyl-DNA phospodiesterase (hTdp1) developed by virtual screening using ligand-based pharmacophores. Bioorg. Med. Chem 2009 Nov. Link
- Marchand, C. et al. Identification of Phosphotyrosine Mimetic Inhibitors of Human Tyrosyl-DNA Phosphodiesterase I by a Novel AlphaScreen High-Throughput Assay. Mol Cancer Ther 8, 240-248 (2009). Link
- Application Support Knowledge Base Home
- AlphaLISA and AlphaScreen No-wash Assays
- Create Your own Alpha Assay
- AlphaLISA Immunoassay Kits
- Alpha Protein-protein and Protein-nucleic Acid Interactions
- Cell-based Protein-protein Interaction Assays
- Alpha Kinase Assays
- Alpha cAMP Assays
- Alpha Epigenetics Assays
- Alpha EMSA Conversion
- Alpha Antibody Detection and Characterization
- Alpha Immunogenicity
- Alpha Ligand-Receptor Binding Assay
- Alpha Protease Assays
- Other Alpha Applications
- Determining Kd With an Alpha Assay
- The Hook Effect
- Bead Selection and Bead Interference
- Buffer Selection for Alpha Assays
- Working with Cell Extracts and Supernatants in Alpha Assays
- Working with Serum and Other Biological Matrices in Alpha Assays
- Alpha Products and Catalog Numbers
- Alpha Citations
- Alpha Troubleshooting Tables