Glycine and bead interference 爱游戏平台注册登录

Glycine and bead interference

The table below shows results from a compound interference study. We tested a panel of 24 compounds in a QC assay using our AlphaLISA^® toolbox bead products. The information below can be used for guidance as to whether glycine might interfere in assays involving particular bead products, and at what glycine concentration interference might occur. These data are derived from single experiments and should serve more as a guide rather than a precise value, as it is unlikely you will be using the exact same assay design as used to generate the data below. Interference concentrations may vary depending on the assay components. It is possible your assay will tolerate higher or lower concentration than what is shown.

		Tested up to 0.1M
Catalog Number	Product	No effect (10%)	50% loss	% of inhibition at max conc
AL125	Streptavidin AlphaLISA Acceptor Beads	1.9E-06	>0.01	15
AL126	Protein L AlphaLISA Acceptor Beads	>0.01	>0.01	0
AL127	Anti-FITC AlphaLISA Acceptor Beads	9.5E-03	>0.01	24
AL128 (biotin)	Anti-6xHis AlphaLISA Acceptor Beads	8.4E-03	9.4E-03	96
AL128 (GSH)	Anti-6XHis AlphaLISA Acceptor Beads	4.0E-03	6.0E-03	81
AL129	Anti-V5 AlphaLISA Acceptor Beads	>0.01	>0.01	0
AL130	Anti-mouse IgM AlphaLISA Acceptor Beads	>0.01	>0.01	0
AL131	Anti-chicken IgY AlphaLISA Acceptor Beads	4.1E-03	>0.01	26
AL132	Anti-sheep IgG AlphaLISA Acceptor Beads	>0.01	>0.01	0
AL133	Anti-GFP AlphaLISA Acceptor Beads	>0.01	>0.01	0
AL134	Anti-MBP AlphaLISA Acceptor Beads	>0.01	>0.01	0
AL136	Strep-Tactin AlphaLISA Acceptor Beads	>0.01	>0.01	0
AS102	Protein A Alpha Donor Beads	2.6E-03	7.3E-03	81
AS103	Anti-FLAG Alpha Donor Beads	>0.01	>0.01	0
AS104	Anti-mouse IgG Alpha Donor Beads	>0.01	>0.01	0
AS105	Anti-rabbit IgG Alpha Donor Beads	1.9E-03	>0.01	15
AS106	Strep-Tactin Alpha Donor Beads	>0.01	>0.01	0
AL900	AlphaLISA TruHits kit 2 ug/mL	>0.01	>0.01	0
AL900	AlphaLISA TruHits kit 20 ug/mL	>0.01	>0.01	0

Refer to the figure below for more detail as to what the values in the table above indicate. Please note that the figure is provided as a general example to explain how the values in our interference tables were determined. The table above indicates that an assay testing the effect of glycine using anti-6X His AlphaLISA Acceptor beads (with glutathione Donor beads) showed little effect on the assay signal at final glycine concentrations up to 4.0 mM. Glycine did show a half-maximal inhibition of signal when used at a final concentration of 6.0 mM. When 0.01 M glycine was included in the assay, 81% signal inhibition was observed.

Figure: Key for how the values in our interference tables are derived. The “no effect” concentration was obtained by extrapolating the MAX - 10% MAX counts. Refer to the table above for actual interference values for each toolbox bead product.