Receptor activation

1. Qi, T. et al. Receptor activity-modifying protein dependent impairment of calcitonin receptor splice variant Δ(1-47)hCT((a)) function. Br. J. Pharmacol. (2012).
2. Hudson, B. D. et al. Chemically engineering ligand selectivity at the free fatty acid receptor 2 based on pharmacological variation between species orthologs. FASEB J. (2012).
3. Fosgerau, K. et al. The novel GLP-1-gastrin dual agonist, ZP3022, increases β-cell mass and prevents diabetes in db/db mice. Diabetes Obes Metab (2012).
4. Lin, D. et al. Identification and Pharmacological Characterization of Multiple Allosteric Binding Sites on the FFA1 Receptor. Mol. Pharmacol. (2012).
5. Gregory, K. J. et al. Investigating mGlu5 Allosteric Modulator Cooperativity, Affinity and Agonism: Enriching Structure-function Studies and Structure-activity Relationships. Mol. Pharmacol. (2012).
6. Avlani, V.A. et al. Critical Role for the Second Extracellular Loop in the Binding of Both Orthosteric and Allosteric G Protein-Coupled Receptor Ligands. J Biol Chem 282, 25677-25686 (2007). Link 
7. Crouch, M.F. & Osmond, R.I.W. New Strategies in Drug Discovery for GPCRs: High Throughput Detection of Cellular ERK Phosphorylation. Comb Chem High Throughput Screen 11, 344-356 (2008). Link 
8. Heinrich, J.N. et al. Aplindore (DAB-452), a High Affinity Selective Dopamine D2 Receptor Partial Agonist. Eur J Pharmacol 552, 36-45 (2006). Link 
9. Lee, H.J. et al. Allosteric Activation of the Extracellular Ca2+-Sensing Receptor by L-Amino Acids Enhances ERK1/2 Phosphorylation. Biochem J 404, 141-149 (2007). Link 
10. Leroy, D. et al. G Protein-Coupled Receptor-Mediated ERK1/2 Phosphorylation: Towards a Generic Sensor of GPCR Activation. J Recept Signal Transduct Res 27, 83-97 (2007). Link 
11. Lodge, A.P., Langmead, C.J., Daniel, G., Anderson, G.W. & Werry, T.D. Performance of Mouse Neural Stem Cells as a Screening Reagent: Characterization of PAC1 Activity in Medium-Throughput Functional Assays. J Biomol Screen (2009). Link
12. May, L.T. et al. Structure-Function Studies of Allosteric Agonism at M2 Muscarinic Acetylcholine Receptors. Mol Pharmacol 72, 463-476 (2007). Link 
13. Morfis, M. et al. Receptor Activity-Modifying Proteins Differentially Modulate the G Protein-Coupling Efficiency of Amylin Receptors. Endocrinology 149, 5423-5431 (2008). Link 
14. Nawaratne, V. et al. New Insights into the Function of M4 Muscarinic Acetylcholine Receptors Gained Using a Novel Allosteric Modulator and a DREADD (Designer Receptor Exclusively Activated by a Designer Drug). Mol Pharmacol 74, 1119-1131 (2008). Link 
15. Osmond, R.I.W. et al. GPCR Screening via ERK 1/2: A Novel Platform for Screening G Protein-Coupled Receptors. J Biomol Screen 10, 730-737 (2005). Link 
16. Siehler, S. Cell-Based Assays in GPCR Drug Discovery. Biotechnol J 3, 471-483 (2008). Link 
17. Tang, Y. et al. Enhancement of Arachidonic Acid Signaling Pathway by Nicotinic Acid Receptor HM74A. Biochem Biophys Res Commun 345, 29-37 (2006). Link 
18. Valant, C. et al. A Novel Mechanism of G Protein-Coupled Receptor Functional Selectivity. Muscarinic Partial Agonist McN-A-343 as a Bitopic Orthosteric/Allosteric Ligand. J Biol Chem 283, 29312-29321 (2008). Link 
19. van der Westhuizen, E.T., Werry, T.D., Sexton, P.M. & Summers, R.J. The Relaxin Family Peptide Receptor 3 Activates Extracellular Signal-Regulated Kinase 1/2 Through a Protein Kinase C-Dependent Mechanism. Mol Pharmacol 71, 1618-1629 (2007). Link 
20. Werry, T.D. et al. Pharmacology of 5HT(2C) Receptor-Mediated ERK1/2 Phosphorylation: Agonist-Specific Activation Pathways and the Impact of RNA Editing. Biochem Pharmacol 76, 1276-1287 (2008). Link

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Signaling pathway studies

1. Luster, T. A. et al. Fusion Toxin BLyS-Gelonin Inhibits Growth of Malignant Human B Cell Lines In Vitro and In Vivo. PLoS ONE 7, e47361 (2012).
2. Brandvold, K. R., Steffey, M. E., Fox, C. C. & Soellner, M. B. Development of a Highly Selective c-Src Kinase Inhibitor. ACS chemical biology (2012).
3. Arner, P. et al. FGF21 Attenuates Lipolysis in Human Adipocytes - a Possible Link to Improved Insulin Sensitivity. FEBS Lett 582, 1725-1730 (2008). Link 
4. Binder, C. et al. Optimization and Utilization of the SureFire Ohospho-STAT5 Assay for a Cell-Based Screening Campaign. Assay Drug Dev Technol 6, 27-37 (2008). Link
5. Chong, M.P., Barritt, G.J. & Crouch, M.F. Insulin Potentiates EGFR Activation and Signaling in Fibroblasts. Biochem Biophys Res Commun 322, 535-541 (2004). Link 
6. Emery, B. et al. SOCS3 Negatively Regulates LIF Signaling in Neural Precursor Cells. Mol Cell Neurosci 31, 739-747 (2006). Link 
7. Hay, D.L. Measurement of Phosphorylated Extracellular Signal-Regulated Kinase 1 and 2 in an Undergraduate Teaching Laboratory with ALPHAscreen Technology. Sci Signal 2, tr1 (2009). Link
8. Roduit, R. & Schorderet, D.F. MAP Kinase Pathways in UV-Induced Apoptosis of Retinal Pigment Epithelium ARPE19 Cells. Apoptosis 13, 343-353 (2008). Link

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爱游戏平台注册登录 Technical Publications

1. Simultaneous Detection of Total and Phosphorylated Protein in Cellular Kinase Assays using Alpha SureFire Ultra Multiplex Technology. Application note (2016). Link
2. Detection and Modulation of Extracellular Matrix Protein Deregulation in Human Prostate Carcinoma Cells Using AlphaLISA Technology. Application note (2016). Link
3. Hurt, S., Titus, D. A Practical Approach to Cell Signaling Pathway Analysis, from sensitive western blots to quantitative assays of multi-target pathways using AlphaScreen® SureFire® assays. Application note (2010). Link 
4. Crouch, M.F., Dyer, A., Brown, A. & Osmond, R.I.W. High throughput cell-based screening strategies for inflammatory receptors. TGR BioSciences Poster (2006). Link
5. Crouch, M.F., Brown, A., Dyer, A. & Osmond, R.I.W. High throughput cell-based screening of TNF4 receptor inhibitors by homogeneous measurement of p38 MAP kinase phosphorylation. TGR BioSciences Poster. Link
6. Dupriez, V., Osmond, R.I.W., Martin-Harris, H., Hohenauer, K. & Park, J. Receptor-Mediated MAPK and Akt Signaling are Maintained in Gamma-irradiated FroZen Cells. Poster by 爱游戏平台注册登录 and TGR BioSciences. Link
7. Hurt, S. & Titus, D. Rapid multi-parameter optimization of AlphaScreen SureFire cellular kinase assays using the JANUS automated workstation. 爱游戏平台注册登录 Poster. Link

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