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Application Brief

HPLC Analysis of Common Ginsenosides Found in Ginseng Using the Chinese Pharmacopeia

Introduction

The term ginseng can refer to several natural products and herbal remedies usually produced from the plant, panax ginseng. Panax ginseng is considered a traditional Chinese medicine (TCM) as it has a reported wide range of medicinal properties such as antioxidant effects and promotion of immune function. The chemistry of ginseng is still not fully understood and more research is required to understand its functions as it becomes increasingly popular in western culture. The main active components of ginseng are ginsenosides of which there are over 100 in various forms of panax ginseng. Ginsenosides are named as Ginsenoside Rx, with x representing the movement on a thin layer chromatography plate, x represents a letter from a-h with a being the most polar and h being the least. This application brief describes the use of an Epic C18 to analyze three ginsenosides using a method based on the Chinese Pharmacopeia (ChP).

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