Spinning disk confocal microscopy is a common tool for live cell microscopy and reduces background fluorescence from out of focus planes. However, for 3D imaging, confocal image quality can be limited by a phenomenon known as ‘pinhole crosstalk’ which can lead to blurred images in conventional spinning disk systems. The confocal scanning unit of the Opera Phenix™ High-Content Screening System provides an increased distance between pinholes to reduce the pinhole crosstalk, leading to improved 3D image quality. Learn more in our Technical Note.