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Determination of Total Protein Using the LAMBDA UV/Vis Spectrophotometer

Application Note

The Determination of Total Protein Using the LAMBDA UV/Vis Spectrophotometer: Bradford Method

Determination of Total Protein Using the LAMBDA UV/Vis Spectrophotometer

Introduction

Quantification methods for total protein are among the longest-established fundamental and important experiments of bioscience. UV/Vis Spectrophotometry is widely used for the determination of protein. This application note describes a typical protein method, the Bradford method. Data is rapidly acquired using the LAMBDA™ 465 UV/Vis Spectrophotometer and processed using the UV Lab Software.

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