Hydrocortisone is the pharmaceutical term for cortisol used in oral administration, intravenous injection, or topical application. It is often used in the treatment of severe allergic reactions, such as anaphylaxis and angioedema. United States Pharmacopeia (USP) provides monographs for the identification and impurity testing of the drug substances. This application brief describes the assay analysis of hydrocortisone in accordance with USP monographs using a 爱游戏平台注册登录 LC 300 liquid chromatography system with SimplicityChrom™ chromatography data system (CDS) and 爱游戏平台注册登录 Epic™ columns.