Mitochondrial dynamics are essential for energy conversion and neuron survival. Understanding changes in mitochondrial dynamics is therefore crucial in developing mitochondria-based therapy options for complex pathological conditions such as cancer, neurological disorders, and metabolic syndromes. Fast kinetic live-cell imaging combined with high-content screening represents a promising strategy to quantitatively track these changes in real time and at large scale.

This application note demonstrates how to investigate mitochondrial dynamics in human iPSC-derived neurons using the Operetta^® CLS^™ high-content analysis system.

• Fast frame rate imaging to accurately capture rapid cellular responses
• Reliable quantification of mitochondrial dynamics in neurons
• Flexible kinetic acquisition settings for each channel to avoid unnecessary data acquisition