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Application Note

Microcapillary electrophoresis analysis of asymmetrical bispecific antibody products using the LabChip GXII Touch

Introduction

In the area of next-generation antibody therapeutics, bispecific antibodies (bsAb) with their exclusive dual binding functions, predominate in the market. The wide diversity of their formats is only limited by our imagination. Therefore, ensuring quality assessment and characteristic analysis has become a real challenge, especially in cell line development in view of isolating clones expressing the right molecule. Among the different subclasses of bsAbs, asymmetrical molecules are of particular interest as they offer the possibility to discriminate the unwanted forms – homodimers – from the active form – heterodimer – by the size.

The LabChip® GXII Touch system, which offers fast-automated analysis and quantitation of protein samples, provides information on concentration and molecular weight, as well as percent purity, charge variants or glycan profiling. Practically no development is required as different application assays are already available as chip kits.

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