CDK Signaling in Oncology: Evaluate Phosphorylation Events Trigged by DNA Damage

DNA double-strand breaks, like those induced by the widely used chemotherapy drug etoposide, trigger a DNA damage response that activates a variety of repair mechanisms, including the phosphorylation of the histone protein component H2AX. DNA damage can directly affect cell cycle progression by modulating levels of an important cell cycle regulator – cyclin-dependent kinase 1 (CDK1).

In this application note, you will learn how to:

• Evaluate DNA damage induced by etoposide treatment using phosphorylated (Ser139) histone H2AX as a biomarker for the detection of DNA double-strand breaks
• Utilize AlphaLISA^® SureFire^® Ultra™ technology to characterize changes in crucial cell cycle events by measuring both phospho- and total target proteins from the same cell lysate sample