In this work, we examine a streamlined methodology for filter homogenization utilizing the OMNI Bead Ruptor Elite™ Bead Mill Homogenizer followed by automated RNA extraction on the chemagic™ Prime™ instrument in preparation for RT-PCR detection of SARS-CoV-2 spiked wastewater samples. This high throughput methodology provides a robust and reproducible workflow for detection of SARS-CoV-2 RNA in wastewater samples.