Rapid and automated electrophoresis is an important tool for research and development of new biotherapeutics. The LabChip GXII Touch Biologics Characterization System performs automated microfluidic capillary electrophoresis on reusable chips with analysis times up to 65 seconds per sample. Microfluidic capillary electrophoresis provides industry leading throughput with accurate sizing and quantitation results. Sample types including cell free DNA, genomic DNA, RNA and proteins may all be characterized on the LabChip system by running the appropriate reagent kit and software package.
The new LabChip Small RNA assay was developed for analysis of small RNAs 20-150 nucleotides (nt) long. The low molecular weight size range can be used for CRISPR/Cas workflows as a quality control check point of synthetic guide RNA (gRNA). The assay also simplifies purity analysis of RNA-targeting therapeutics. Like slab gel electrophoresis protocols published for small RNA analytes that use high agarose cross-linking content and high concentration of urea, the new LabChip Small RNA assay uses a urea containing gel formation with pore size optimized for small RNA detection. The GXII Reviewer settings for small RNA analysis allows fragment analysis of small RNA molecules analogous to the method used for LabChip High Sensitivity DNA assays.
Note; This assay is compatible with both the LabChip GX and LabChip GXII systems.