ELISA assays are widely used for the detection of aflatoxins M1 in milk for regulatory conformance owing to the high sensitivity, selectivity, and ease of use of the method. In the following study we demonstrate the accuracy and precision of the MaxSignal® Aflatoxin M1 ELISA kit at the EU MRL of 0.05 ppb and the US MRL of 0.5 ppb. We also provide data showing the limit of detection (LOD) of the kit. Further, we show the variability between performance of manual ELISA and DS2 automated ELISA platform.