A Workflow to Characterize and Benchmark Human Induced Pluripotent Stem Cells

The UK-based Human Induced Pluripotent Stem Cell Initiative (HipSci) aims to offer the scientific community access to a vast panel of cell lines with thorough characterization and data analysis tools.

This case study details a phenotypic screen to characterize human iPSCs on diverse extracellular matrix substrates, and a method for the capture of specific phenotypes emerging upon cell-to-cell contact.

This case study describes:

1. How high-content analysis enables image-based phenotyping and the throughput for analysis of hundreds of cell lines.
2. How data analysis tools help understand multiparametric datasets through interactive data visualization and phenotype classification.