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Adenosine A2A AequoScreen; cell line

Stable, recombinant AequoScreen® cell line expressing aequorin and the Adenosine A2A receptor, human recombinant in HEK293 host cells.

Part Number ES-011-A
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Cell Line Terms and Conditions must be accepted before orders are placed. Additional agreement needed for commercial use.

Overview

爱游戏平台注册登录 ’s AequoScreen® double transfected cell lines are provided in two vials of frozen cells, each containing ∽2.5 x 106 cells. Detailed product information as well as recommended cell culture conditions and validation assay date are available for each cell line.

The AequoScreen technology is a generic GPCR technology that can be used with Gs, Gi, and Gq-coupled GPCRs and calcium coupled ion channels. Following receptor stimulation, increases in intracellular calcium enable measurement of the resulting flash luminescence signal.

Features:

  • Fully automatable and miniaturizable to 1536
  • Enables reduction in reagents and consumable costs
  • High signal to background ratio enabling detection of agonists, antagonists and allosteric modulators
  • Robust assay (Z′, CV values, solvent resistance)
  • Reduces the number of false positives from autofluorescent compounds in screening libraries
  • Option to work with cells in suspension to increase throughput
  • Amenable for use with calcium coupled ion channels and tyrosine kinase receptors

爱游戏平台注册登录 ’s AequoScreen cell lines require accepted cell line Terms and Conditions prior to order processing. Additional agreement needed for commercial use. Contact us using the Request More Information button above for additional information regarding required documentation.

Specifications

Assay Target Class GPCR
Assay Target Type Cell line
Assay Validated Calcium Luminescence, Binding, Calcium Fluorescence
G-Alpha Coupling Protein Ga16
G-Alpha Natural Receptor Gs
Host HEK293
Product Brand Name AequoScreen
Receptor Type Adenosine
Second Messenger Release Calcium flux
Shipping Condition Dry Ice
Special Ordering Information Requires accepted Terms and Conditions. Additional agreement needed for commercial use.
Therapeutic Area Central Nervous System, Pain, Cardiovascular, Inflammation
Unit Size 5 million cells
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Brochure

GPCRs: The Pathway to Discovery

G protein coupled-receptors (GPCRs) are one of the most intensively studied drug targets, with up to one-third of all marketed therapeutics acting by binding to GPCRs. Therapies that target GPCRs include both agonist and antagonists that are used in the treatment of disease in nearly every major ...

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Radioligand Selection Guide

Discover a comprehensive collection of GPCR cell lines, membrane preparations, and complementary radioligands designed to drive your GPCR research. Utilize this selection guide to easily identify:

  • Cell lines available for specific receptors
  • Assays that cell lines are validat ...

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Take the Stress Out of Functional Testing - AequoZen and cAMPZen ready-to-use cells

爱游戏平台注册登录 ’s validated, ready-to-use AequoZen™or cAMPZen™ frozen, irradiated cells make it easier for you to perform functional testing of GPCRs. Just thaw and use! Reliable, convenient AequoZen cells for aequorin calcium testing or cAMPZen cells for cAMP testing let you get the testing done. So yo ...

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Manual

AequoZen Frozen Cells Starter Kit

G protein coupled receptors (GPCRs) have been considered as a highly “druggable” target for many years, with over 40% of marketed drugs acting to modulate their function. For many years, radiometric techniques have dominated GPCR screening. However in the last decade the development of functional as ...

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