Stable, recombinant AequoScreen® cell line expressing aequorin and the α2A Adrenergic receptor, human recombinant in CHO-K1 host cells.
false falseCell Line Terms and Conditions must be accepted before orders are placed. Additional agreement needed for commercial use.
爱游戏平台注册登录 ’s AequoScreen® double transfected cell lines are provided in two vials of frozen cells, each containing ∽2.5 x 106 cells. Detailed product information as well as recommended cell culture conditions and validation assay date are available for each cell line.
The AequoScreen technology is a generic GPCR technology that can be used with Gs, Gi, and Gq-coupled GPCRs and calcium coupled ion channels. Following receptor stimulation, increases in intracellular calcium enable measurement of the resulting flash luminescence signal.
Features:
爱游戏平台注册登录 ’s AequoScreen cell lines require accepted cell line Terms and Conditions prior to order processing. Additional agreement needed for commercial use. Contact us using the Request More Information button above for additional information regarding required documentation.
檢測目標類 | GPCR |
---|---|
檢測目標類型 | Cell line |
檢測驗證 | Binding, Calcium Luminescence, Calcium Fluorescence |
G-Alpha偶聯蛋白 | Ga16 |
G-Alpha天然受體 | Gi/Go |
宿主細胞 | CHO-K1 |
產品品牌名稱 | AequoScreen |
受體類型 | Adrenergic |
第二信使釋放 | Calcium flux |
運輸條件 | 幹冰 |
特殊訂購信息 | Requires accepted Terms and Conditions. Additional agreement needed for commercial use. |
治療領域 | Cardiovascular, Inflammation, Metabolic, Diabetes, Gastrointestinal |
產品尺寸 | 5 million cells |
G protein coupled-receptors (GPCRs) are one of the most intensively studied drug targets, with up to one-third of all marketed therapeutics acting by binding to GPCRs. Therapies that target GPCRs include both agonist and antagonists that are used in the treatment of disease in nearly every major ...
Discover a comprehensive collection of GPCR cell lines, membrane preparations, and complementary radioligands designed to drive your GPCR research. Utilize this selection guide to easily identify:
Aequorin and Photina cell lines – the alternative calcium flux assay. PDL-coated microplates improve your performance
Aequorin is a photo protein originating from the jellyfish Aequorea Victoria. The apo-enzyme (apoaequorin) is a 21 kD protein, which requires a hydrophobic prosthetic group, coelenterazine, to be converted to aequorin,the active form of the enzyme. This enzyme possesses 3 calcium binding sites which ...
We have shown here that signal intensity for the selected catalog aequorin cell lines is strong enough to allow its measurement by the non-luminescence dedicated FLIPR