爱游戏平台注册登录
check quantity

AlphaLISA Mouse IL-18 Detection Kit, 100 Assay Points

The AlphaLISA® IL-18 Detection Kit is designed for the quantitative determination of mouse IL-18 in serum, buffered solution, or cell culture medium using a homogeneous (no wash steps, no separation steps) assay.

For research use only; not for diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).

貨號
產品尺寸
AL3189HV
100 Assay Points
更多
AL3189C
500 Assay Points
更多
AL3189F
5,000 Assay Points
更多
Request Technical Support

請輸入有效數量

請登錄以添加收藏夾。

購物車為空

詳細信息

Interleukin-18 (IL-18), also known as interferon-gamma inducing factor, is a proinflammatory cytokine of the IL-1 family that is primarily secreted by macrophages. IL-18’s biological activity resembles that of IL-12, with the ability to enhance NK cell activity and induce IFNγ secretion by PMBC. A key role of IL-18 is its ability to eliminate VEGF from the eye to protect the retina. In combination with anti-VEGF antibodies, IL-18 is utilized to treat age-related macular degeneration (AMD). IL-18 dysregulation is also linked to severe inflammatory reactions.

Formats

  • Our 100 assay point kit allows you to run 100 wells in 96-well format, using a 100 µL reaction volume (10 µL of sample).
  • Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
  • Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

Features

  • No-wash steps, no separation steps
  • ELISA alternative technology
  • Sensitive detection
  • Broad sample compatibility
  • Small sample volume
  • Results in less than 3 hours
  • Half the time of an ELISA assay

AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

規格

檢測目標 IL-18
檢測技術 Alpha
自動化兼容 Yes
檢測方法 Alpha
實驗類型 In vitro
產品品牌名稱 AlphaLISA
運輸條件 藍冰
Target Species Mouse
產品尺寸 100 Assay Points
資源
  • 所有

產品手冊

Drug Discovery Screening Solutions Brochure

Find out about our range of integrated solutions for drug discovery screening in this e-brochure.

Our screening solutions for high-throughput screening, phenotypic screening and data analysis help to streamline drug discovery workflows in labs across the globe. Our portfolio includes automat ...

PDF 4 MB
Alpha Technology Solutions

Product brochure for the Alpha Technology, a versatile, no wash, homogeneous assay technology that's suitable for a broad range of applications.

PDF 2 MB

應用文獻

Applicability of AlphaLISA Technology to a Wide Spectrum of Complex Biological Samples

Alpha (Amplified Luminescent Proximity Homogeneous Assay) technology is a bead-based, no-wash alternative to traditional ELISAs. Instead of detection with an HRP substrate, the Alpha assay signal is generated by the excitation of an Eu+-coated bead that has been ...

PDF 1 MB

應用簡報

Eight Limitations of ELISA and How to Overcome Them Using Alternative Technologies

The introduction of enzyme-linked immunosorbent assays (ELISAs) in the early 1970’s offered researchers a non-radiometric immunoassay platform without compromising sensitivity. Over the last 50 years scientists have made huge strides in disease research and drug discovery and a demand for greater ...

PDF 1 MB

數據單表

AlphaLISA Mouse Interleukin-18 Detection Kit

AlphaLISA Mouse Interleukin-18 Detection Kit

PDF 462 KB

海報

All-In-One-Well AlphaLISA Assays for Direct Biomarker Quantification in Cell Cultures

Biomarker levels were measured directly in CulturPlates-96 and-384 in a simple, fast, all-in-one-well AlphaLISA assay format. The elimination of transfer and wash steps simplifies cellular assays, reduces variability and significantly reduces hands-on time and costs associated with consumables. Int ...

PDF 288 KB
AlphaLISA Assays are Homogeneous Sensitive Immunoassays for Detection of Analytesin a Variety of Biological Matrices

The AlphaLISA® assay is a homogeneous immunoassay alternative to classical ELISA. AlphaLISA assays were originally utilized to detect analytesin cell cultures upernatants or serum/plasma samples.

PDF 273 KB
Baidu
map