For research use only; not for diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Interleukin-18 (IL-18), also known as interferon-gamma inducing factor, is a proinflammatory cytokine of the IL-1 family that is primarily secreted by macrophages. IL-18’s biological activity resembles that of IL-12, with the ability to enhance NK cell activity and induce IFNγ secretion by PMBC. A key role of IL-18 is its ability to eliminate VEGF from the eye to protect the retina. In combination with anti-VEGF antibodies, IL-18 is utilized to treat age-related macular degeneration (AMD). IL-18 dysregulation is also linked to severe inflammatory reactions.
AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.
檢測目標 | IL-18 |
---|---|
檢測技術 | Alpha |
自動化兼容 | Yes |
檢測方法 | Alpha |
實驗類型 | In vitro |
產品品牌名稱 | AlphaLISA |
運輸條件 | 藍冰 |
Target Species | Mouse |
產品尺寸 | 100 Assay Points |
Find out about our range of integrated solutions for drug discovery screening in this e-brochure.
Our screening solutions for high-throughput screening, phenotypic screening and data analysis help to streamline drug discovery workflows in labs across the globe. Our portfolio includes automat ...
Product brochure for the Alpha Technology, a versatile, no wash, homogeneous assay technology that's suitable for a broad range of applications.
Alpha (Amplified Luminescent Proximity Homogeneous Assay) technology is a bead-based, no-wash alternative to traditional ELISAs. Instead of detection with an HRP substrate, the Alpha assay signal is generated by the excitation of an Eu+-coated bead that has been ...
The introduction of enzyme-linked immunosorbent assays (ELISAs) in the early 1970’s offered researchers a non-radiometric immunoassay platform without compromising sensitivity. Over the last 50 years scientists have made huge strides in disease research and drug discovery and a demand for greater ...
AlphaLISA Mouse Interleukin-18 Detection Kit
Biomarker levels were measured directly in CulturPlates-96 and-384 in a simple, fast, all-in-one-well AlphaLISA assay format. The elimination of transfer and wash steps simplifies cellular assays, reduces variability and significantly reduces hands-on time and costs associated with consumables. Int ...
The AlphaLISA® assay is a homogeneous immunoassay alternative to classical ELISA. AlphaLISA assays were originally utilized to detect analytesin cell cultures upernatants or serum/plasma samples.