AlphaLISA SureFire Ultra Human Total CDK7 Detection Kit, 500 Assay Points

Cyclin-dependent kinase 7 (CDK7), or cell division protein kinase 7, is encoded by the CDK7 gene which is a member of the CDK family. CDK7 has dual functions, as a CDK–activating kinase (CAK) that regulates cell cycle progression by phosphorylation of CDK1, CDK2, and CDK4/6, and as a component of transcription factor II H (TFIIH) that regulates transcriptional initiation through phosphorylation of RNA polymerase II C-terminal domain. Inhibition of CDK7 offers an opportunity for restoring controlled cell cycle through regulating multiple cell cycle checkpoints, making it a cancer therapy target.

The AlphaLISA SureFire Human Total CDK7 Detection Kit is a sandwich immunoassay for the quantitative detection of total CDK7 in cellular lysates, using Alpha Technology.

Formats

• The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.
• The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.
• The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.
• The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.

AlphaLISA SureFire Ultra kits are compatible with

• Cell and tissue lysates
• Antibody modulators
• Biotherapeutic antibodies

Alpha SureFire kits can be used for

• Cellular kinase assays
• Receptor activation studies
• Screening