AlphaLISA SureFire Ultra Human Total Cyclin E1 Detection Kit, 100 Assay Points

Cyclin E1 (CCNE1) is a member of the highly conserved cyclin family, whose members have various roles in the cell cycle. CCNE1 forms a complex with cyclin-dependent kinase 2 (CDK2) and acts as a regulatory subunit. CDK2 is required for cell cycle G1/S transition. Overexpression of CCNE1 has been observed in many tumors, such as breast, ovarian, endometrial, and bladder cancer.

The AlphaLISA SureFire Human Total Cyclin E1 Detection Kit is a sandwich immunoassay for the quantitative detection of total Cyclin E1 in cellular lysates, using Alpha Technology.

Formats

• The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.
• The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.
• The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.
• The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.

AlphaLISA SureFire Ultra kits are compatible with

• Cell and tissue lysates
• Antibody modulators
• Biotherapeutic antibodies

Alpha SureFire kits can be used for

• Cellular kinase assays
• Receptor activation studies
• Screening