Phospho DAP12 (pan Tyr) Alpha SureFire Ultra Detection kit, 500

DAP12, also known as TYRO protein tyrosine kinase-binding protein, is a signaling adaptor for several cell surface receptors of the innate immune system. Crosslinking receptors associated with DAP12 leads to phosphorylation of tyrosine residues in their cytoplasmic ITAM by SRC family kinases, and initiates downstream signaling through the activation of SYK or Zap-70 and additional downstream targets. The TREM2-DAP12 receptor complex appears to play a continuous surveying role by scanning the neuronal microenvironment and maintaining a healthy brain homeostasis in Microglia cells.

The AlphaLISA^® SureFire^® Ultra^™ p-DAP12 (pan Tyr) assay is a sandwich immunoassay for the quantitative detection of phospho-DAP12 (phosphorylated on Tyr) in cellular lysates, using Alpha Technology.”

 Formats:

• The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.
• The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.
• The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.
• The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.

AlphaLISA SureFire Ultra kits are compatible with:

• Cell and tissue lysates
• Antibody modulators
• Biotherapeutic antibodies

Alpha SureFire kits can be used for:

• Cellular kinase assays
• Receptor activation studies
• Screening