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AlphaLISA SureFire Ultra p-ERK1/2 (Thr202/Tyr204) Assay Kit - 500 Assay Points

The AlphaLISA® SureFire® Ultra p-ERK 1/2 (Thr202/Tyr204) assay is a sandwich immunoassay for quantitative detection of phospho-ERK 1/2 (phosphorylated on Thr202/Tyr204 in ERK1, or Thr185/Tyr187 in ERK2) in cellular lysates using Alpha Technology.

貨號
產品尺寸
ALSU-PERK-A-HV
100 Assay Points
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ALSU-PERK-A500
500 Assay Points
更多
ALSU-PERK-A10K
10,000 Assay Points
更多
ALSU-PERK-A50K
50,000 Assay Points
更多
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詳細信息

Formats:

  • The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 µL reaction volume.
  • The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 µL reaction volume.
  • The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 µL reaction volume.
  • The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 µL reaction volume.

In the AlphaLISA® SureFire® Ultra assay, Donor beads are coated with streptavidin to capture one of the antibodies, which is biotinylated. Acceptor beads are coated with a proprietary CaptSure agent that immobilizes the other antibody, labeled with a CaptSure tag. In the presence of phosphorylated protein, the two antibodies bring the Donor and Acceptor beads close together, generating signal.  The amount of light emission is directly proportional to the amount of phosphoprotein present in the sample.

AlphaLISA® SureFire® Ultra kits are compatible with:

  • Cell and tissue lysates
  • Antibody modulators
  • Biotherapeutic antibodies

Alpha SureFire® kits can be used for:

  • Cellular kinase assays
  • Receptor activation studies
  • Screening

規格

分析途徑 MAPK
檢測目標 ERK1/2
檢測目標類 Phosphoprotein
自動化兼容 Yes
檢測方法 Alpha
Format Microplates
產品品牌名稱 AlphaLISA SureFire Ultra
包裝量 500.0 Units
運輸條件 藍冰
治療領域 Central Nervous System, Cardiovascular, Neuroinflammation
產品尺寸 500 Assay Points
資源
  • 所有

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