AlphaLISA SureFire Ultra Human and Mouse Phospho-LRRK2 (Ser910) Detection Kit, 500 Assay Points

Leucine-rich repeat kinase 2, also known as LRRK2, Dardarin, or PARK8, is a member of the ROCO superfamily and is a large, multifunctional kinase enzyme. The ROCO family characteristics include the presence of a tandem Ras of complex (Roc) G-domain, kinase domains, and carboxy- terminal of Roc (COR) sequence which links them. LRRK2 modulates Tau phosphorylation via GSK-3b and is involved in lysosomal function and the innate immunity in the brain (microglia activation). Mutations observed in LRRK2 have been associated with Parkinson’s disease.

The AlphaLISA SureFire Human and Mouse Phospho-LRRK2 (Ser910) Detection Kit is a sandwich immunoassay for the quantitative detection of phospho-LRRK2 (Ser910) in cellular lysates, using Alpha Technology.

Formats

• The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.
• The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.
• The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.
• The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.

AlphaLISA SureFire Ultra kits are compatible with

• Cell and tissue lysates
• Antibody modulators
• Biotherapeutic antibodies

Alpha SureFire kits can be used for

• Cellular kinase assays
• Receptor activation studies
• Screening