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Phospho-MLKL (Ser345) (Mouse) AlphaLISA SureFire Ultra Detection Kit, 10,000 Assay Points

The AlphaLISA® SureFire® Ultra mouse Phospho-MLKL (Ser345) assay is a sandwich immunoassay for quantitative detection of Phospho-MLKL (Ser345) in cellular lysates using Alpha Technology.

For research use only; not for diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).

貨號
產品尺寸
ALSU-PMLKL-A500
500 Assay Points
更多
ALSU-PMLKL-A10K
10,000 Assay Points
更多
ALSU-PMLKL-A50K
50,000 Assay Points
更多
ALSU-PMLKL-A-HV
100 Assay Points
更多
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詳細信息

Mixed lineage kinase domain-like protein is encoded by the MLKL gene in murine species. MLKL is a pseudokinase that plays a crucial role in TNF-induced necroptosis. MLKL is activated following phosphorylation by RIP3 kinase, which induces a conformational switch essential for necroptosis. The protein kinase domain of MLKL is catalytically inactive, but contains a pseudoactive site with an interaction between Lys-219 and Gln-343 residues. Upon phosphorylation by RIP3, MLKL undergoes an active conformation and homodimerization through its amino-terminal coiled-coil domain.

The AlphaLISA® SureFire® Ultra™ mouse p-MLKL (Ser345) assay is a sandwich immunoassay for quantitative detection of Phospho-MLKL (Ser345) in cellular lysates using Alpha Technology.

Formats:

  • The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.
  • The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.
  • The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.
  • The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.

AlphaLISA SureFire Ultra kits are compatible with:

  • Cell and tissue lysates
  • Antibody modulators
  • Biotherapeutic antibodies

Alpha SureFire kits can be used for:

  • Cellular kinase assays
  • Receptor activation studies
  • Screening

規格

檢測目標 MLKL
檢測技術 Alpha
自動化兼容 Yes
檢測方法 Alpha
實驗類型 In vitro
產品品牌名稱 AlphaLISA SureFire Ultra
運輸條件 藍冰
治療領域 Neuroinflammation, Oncology, Inflammation
產品尺寸 10,000 Assay Points
資源
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