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This control lysate is intended for use with the AlphaLISA SureFire Ultra Total MLKL Assay Kit, or for use as a control for those running orthogonal or secondary assays along with the SureFire assay. To prepare the lysate, L929 cells were cultured to confluence in T175 flasks in 10% FBS containing medium. Cells were treated with 20 μM Z-VAD, 100 nM SM-164 and 50 ng/mL TNFα for 4 hours at 37°C. Cells were lysed in 5 mL 1X SureFire Ultra Lysis buffer.
| Assay Target | MLKL |
|---|---|
| Assay Technology | Alpha |
| Automation Compatible | Yes |
| Detection Method | Alpha |
| Experimental Type | In vitro |
| Product Brand Name | AlphaLISA SureFire Ultra |
| Shipping Condition | Blue Ice |
| Therapeutic Area | Neuroinflammation, Oncology, Inflammation |
| Unit Size | 1 vial |
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