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AlphaLISA SureFire® Ultra™ phospho-mTOR (Ser2448) Assay Kit, 100 Assay Points

The AlphaLISA® SureFire® Ultra™ phospho-mTOR (Ser2448) assay kit can be used to measure levels of mTOR (phosphorylated at Ser2448) in cellular lysates using no-wash Alpha technology.

For research use only; not for diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).

Part Number
Unit Size
List Price
Your Price
Quantity
ALSU-PMTOR-C-HV
100 Assay Points
662.00 USD
 
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ALSU-PMTOR-C500
500 Assay Points
2236.00 USD
 
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ALSU-PMTOR-C10K
10,000 Assay Points
13600.00 USD
 
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ALSU-PMTOR-C50K
50,000 Assay Points
43900.00 USD
 
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爱游戏平台注册登录 Life Sciences & Diagnostics is now Revvity, but at this time, there is no change to the supplier information.
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Overview

Formats:

  • The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 µL reaction volume.
  • The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 µL reaction volume.
  • The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 µL reaction volume.
  • The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 µL reaction volume.

In the AlphaLISA® SureFire® Ultra™ assay, Donor beads are coated with streptavidin to capture one of the antibodies, which is biotinylated. Acceptor beads are coated with a proprietary CaptSure™ agent that immobilizes the other antibody, labeled with a CaptSure™ tag. In the presence of phosphorylated protein, the two antibodies bring the Donor and Acceptor beads close together, generating signal. The amount of light emission is directly proportional to the amount of phosphoprotein present in the sample.

AlphaLISA® SureFire® Ultra™ kits are compatible with:

  • Cell and tissue lysates
  • Antibody modulators
  • Biotherapeutic antibodies

Alpha SureFire® kits can be used for:

  • Cellular kinase assays
  • Receptor activation studies
  • Screening

Specifications

Assay Pathway PI3K/Akt/mTOR
Assay Target mTOR
Assay Target Class Phosphoprotein
Automation Compatible Yes
Detection Method Alpha
Molecular Modification Phosphorylation
Product Brand Name AlphaLISA SureFire Ultra
Shipping Condition Blue Ice
Therapeutic Area Central Nervous System, Cardiovascular, Metabolic
Unit Size 100 Assay Points
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