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AlphaLISA SureFire® Ultra™ total mTOR Assay Kit, 10,000 Assay Points

The AlphaLISA® SureFire® Ultra™ total mTOR assay kit can be used to measure levels of mTOR (both phosphorylated and non-phosphorylated) in cellular lysates using no-wash Alpha technology.

This kit has been pre-validated for use in combination with Pelago Bioscience’s CETSA® (Cellular Thermal Shift Assay) technology. Please note that further assay optimization will be required in order to adapt the kit to your particular cell line and experimental conditions. A valid CETSA® licence from Pelago Bioscience or a single kit agreement is required for using the CETSA® technology.

For research use only; not for diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).

貨號
產品尺寸
ALSU-TMTOR-B-HV
100 Assay Points
更多
ALSU-TMTOR-B500
500 Assay Points
更多
ALSU-TMTOR-B10K
10,000 Assay Points
更多
ALSU-TMTOR-B50K
50,000 Assay Points
更多
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詳細信息

Formats:

  • The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 µL reaction volume.
  • The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 µL reaction volume.
  • The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 µL reaction volume.
  • The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 µL reaction volume.

In the AlphaLISA® SureFire® Ultra™ assay, Donor beads are coated with streptavidin to capture one of the antibodies, which is biotinylated. Acceptor beads are coated with a proprietary CaptSure™ agent that immobilizes the other antibody, labeled with a CaptSure™ tag. In the presence of target protein, the two antibodies bring the Donor and Acceptor beads close together, generating signal. The amount of light emission is directly proportional to the amount of protein present in the sample.

AlphaLISA® SureFire® Ultra™ kits are compatible with:

  • Cell and tissue lysates
  • Antibody modulators
  • Biotherapeutic antibodies

Alpha SureFire® kits can be used for:

  • Cellular kinase assays
  • Receptor activation studies
  • Screening

規格

分析途徑 PI3K/Akt/mTOR
檢測目標 mTOR
檢測目標類 Protein
自動化兼容 Yes
檢測方法 Alpha
產品品牌名稱 AlphaLISA SureFire Ultra
運輸條件 藍冰
治療領域 Central Nervous System, Cardiovascular, Metabolic
產品尺寸 10,000 Assay Points
資源
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