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AlphaLISA SureFire Ultra Human Phospho-NPM (Thr199) Detection Kit, 10,000 Assay Points

The AlphaLISA® SureFire® Ultra Human Phospho-NPM (Thr199) assay is a sandwich immunoassay for quantitative detection of phospho-NPM (Thr199) in cellular lysates using Alpha Technology.

For research use only; not for diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).

貨號
產品尺寸
ALSU-PNPM-A500
500 Assay Points
更多
ALSU-PNPM-A10K
10,000 Assay Points
更多
ALSU-PNPM-A50K
50,000 Assay Points
更多
ALSU-PNPM-A-HV
100 Assay Points
更多
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詳細信息

Nucleophosmin (NPM, Nucleolar Phosphoprotein B23, No38, or Numatrin) is a highly conserved, abundant nucleolar protein found in the nuclei of proliferating cells. NPM is a chaperone protein that shuttles rapidly between the nucleus and cytoplasm. It plays key roles in ribosome biogenesis, centrosome duplication, genomic stability, cell cycle progression, and apoptosis. NPM overexpression, mutation, rearrangement, or deletion, is associated with several types of cancer.

The AlphaLISA SureFire Human Phospho-NPM (Thr199) Detection Kit is a sandwich immunoassay for the quantitative detection of phospho-NPM (Thr199) in cellular lysates, using Alpha Technology.

Formats

  • The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.
  • The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.
  • The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.
  • The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.

AlphaLISA SureFire Ultra kits are compatible with

  • Cell and tissue lysates
  • Antibody modulators
  • Biotherapeutic antibodies

Alpha SureFire kits can be used for

  • Cellular kinase assays
  • Receptor activation studies
  • Screening

規格

檢測目標 NPM
檢測技術 Alpha
自動化兼容 Yes
檢測方法 Alpha
實驗類型 In vitro
產品品牌名稱 AlphaLISA SureFire Ultra
運輸條件 藍冰
Target Species Human
治療領域 Oncology
產品尺寸 10,000 Assay Points
資源
  • 所有

產品手冊

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Product brochure for the Alpha Technology, a versatile, no wash, homogeneous assay technology that's suitable for a broad range of applications.

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應用簡報

Eight Limitations of ELISA and How to Overcome Them Using Alternative Technologies

The introduction of enzyme-linked immunosorbent assays (ELISAs) in the early 1970’s offered researchers a non-radiometric immunoassay platform without compromising sensitivity. Over the last 50 years scientists have made huge strides in disease research and drug discovery and a demand for greater ...

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