AlphaLISA SureFire Ultra Human and Mouse Total PERK Detection Kit, 500 Assay Points

Protein kinase-like endoplasmic reticulum kinase (PERK) is an eIF2α kinase and transmembrane protein that resides in the endoplasmic reticulum (ER) membrane and is activated upon ER stress signals. PERK activation during ER stress correlates with autophosphorylation of its cytoplasmic kinase domain. Phosphorylation of PERK at Thr980 serves as a marker for its activation status. PERK promotes tumor growth by limiting oxidative DNA damage and is associated with neurodegenerative diseases and many cancers.

The AlphaLISA SureFire Human and Mouse Total PERK Detection Kit is a sandwich immunoassay for the quantitative detection of total PERK in cellular lysates, using Alpha Technology.

Formats

• The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.
• The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.
• The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.
• The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.

AlphaLISA SureFire Ultra kits are compatible with

• Cell and tissue lysates
• Antibody modulators
• Biotherapeutic antibodies

Alpha SureFire kits can be used for

• Cellular kinase assays
• Receptor activation studies
• Screening