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Phospho-RSK1 (Ser380) AlphaLISA SureFire Ultra Detection Kit, 50,000 Assay Points

The AlphaLISA® SureFire® Ultra Phospho-RSK1 (Ser380) assay is a sandwich immunoassay for quantitative detection of Phospho-RSK1 (Ser380) in cellular lysates using Alpha Technology.

For research use only; not for diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).

Part Number
Unit Size
List Price
Your Price
Quantity
ALSU-PRSK1-C500
500 Assay Points
2236.00 USD
 
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ALSU-PRSK1-C10K
10,000 Assay Points
13600.00 USD
 
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ALSU-PRSK1-C50K
50,000 Assay Points
43900.00 USD
 
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ALSU-PRSK1-C-HV
100 Assay Points
706.00 USD
 
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Overview

Ribosomal protein S6 kinases (RSK) are a family of broadly expressed serine/threonine kinases activated in response to mitogenic stimuli, including tumor-promoting growth factors and phorbol esters. RSKs are positioned downstream of the Raf-MEK-ERK signal transduction pathway, and are directly phosphorylated by ERK1 and ERK2extracellular kinases. Active RSKs appear to play a significant role in transcriptional regulation, translocation to the nucleus, and phosphorylation of c-Fos and CREBs.

Current models suggest that RSK activation requires a binding of active ERK1/2 to a docking site at the carboxyl end of RSK. ERK moored to RSK1 phosphorylate at several sites, including the CTD activation loop (carboxyl-terminal kinase domain). The CTD autophosphorylates Serine 380, a site in the central hydrophobic region.

The AlphaLISA® SureFire® Ultra p-RSK1 (Ser380) assay is a sandwich immunoassay for the quantitative detection of phospho-RSK1 (phosphorylated on Ser380) in cellular lysates, using Alpha Technology.

Formats:

  • The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.
  • The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.
  • The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.
  • The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.

AlphaLISA SureFire Ultra kits are compatible with:

  • Cell and tissue lysates
  • Antibody modulators
  • Biotherapeutic antibodies

Alpha SureFire kits can be used for:

  • Cellular kinase assays
  • Receptor activation studies
  • Screening

Specifications

Assay Target Rsk1
Assay Technology Alpha
Automation Compatible Yes
Detection Method Alpha
Experimental Type In vitro
Product Brand Name AlphaLISA SureFire Ultra
Shipping Condition Blue Ice
Therapeutic Area Oncology
Unit Size 50,000 Assay Points
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Application Brief

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Brochure

Alpha Technology Solutions

Product brochure for the Alpha Technology, a versatile, no wash, homogeneous assay technology that's suitable for a broad range of applications.

PDF 2 MB
Drug Discovery Screening Solutions Brochure

Find out about our range of integrated solutions for drug discovery screening in this e-brochure.

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