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Phospho-SHP-2 (Tyr542) AlphaLISA SureFire Ultra Detection Kit, 10,000 Assay Points

The AlphaLISA® SureFire® Ultra™ p-SHP-2 (Tyr542) assay is a sandwich immunoassay for quantitative detection of phospho-SHP-2 (phosphorylated on Tyr542) in cellular lysates using Alpha Technology.

For research use only; not for diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).

貨號
產品尺寸
ALSU-PSHP2-A-HV
100 Assay Points
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ALSU-PSHP2-A500
500 Assay Points
更多
ALSU-PSHP2-A10K
10,000 Assay Points
更多
ALSU-PSHP2-A50K
50,000 Assay Points
更多
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詳細信息

Formats:

  • The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 µL reaction volume.
  • The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 µL reaction volume.
  • The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 µL reaction volume.
  • The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 µL reaction volume.

In the AlphaLISA SureFire Ultra assay, Donor beads are coated with streptavidin to capture one of the antibodies, which is biotinylated. Acceptor beads are coated with a proprietary CaptSure™ agent that immobilizes the other antibody, labeled with a CaptSure™ tag. In the presence of target protein, the two antibodies bring the Donor and Acceptor beads close together, generating signal. The amount of light emission is directly proportional to the amount of protein present in the sample.

AlphaLISA SureFire Ultra™ kits are compatible with:

  • Cell and tissue lysates
  • Antibody modulators
  • Biotherapeutic antibodies

Alpha SureFire kits can be used for:

  • Cellular kinase assays
  • Receptor activation studies
  • Screening

規格

檢測目標 SHP-2
檢測目標類 Phosphoprotein
檢測方法 Alpha
產品品牌名稱 AlphaLISA SureFire Ultra
運輸條件 藍冰
產品尺寸 10,000 Assay Points
資源
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