SMAD family member 4 (SMAD4), also known as mothers against decapentaplegic homolog 4, or DPC4 (deleted in pancreatic cancer-4), belongs to the SMAD family of transcription factor proteins, which act as mediators of TGF-β signal transduction. In conjunction with SMAD 2 and SMAD 3, the SMAD2/3/4 complex forms and acts as a transcription factor that promotes the expression of a pro-fibrotic phenotype. Mutated SMAD4 is found in many types of cancers and fibrosis.

The AlphaLISA SureFire Ultra Human and Mouse Total SMAD4 Detection Kit is a sandwich immunoassay for the quantitative detection of total SMAD4 in cellular lysates, using Alpha Technology.

Formats

• The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.
• The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.
• The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.
• The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.

AlphaLISA SureFire Ultra kits are compatible with

• Cell and tissue lysates
• Antibody modulators
• Biotherapeutic antibodies

Alpha SureFire kits can be used for

• Cellular kinase assays
• Receptor activation studies
• Screening