AlphaLISA SureFire Ultra Human and Mouse Total SMARCA4 Detection Kit, 50,000 Assay Points

Brahma-related gene 1 (BRG1), also known as SMARCA4, is an ATP-dependent chromatin remodeler encoded by the SMARCA4 gene. It is the central ATPase catalytic subunit of the switch/sucrose nonfermentable (SWI/SNF) chromatin remodeling complex, which plays a core role in DNA replication, repair, recombination, and transcriptional regulation. The SWI/SNF chromatin remodeling complex is mutated in 20% of all cancers and SMARCA4 is aberrantly expressed in various cancers.

The AlphaLISA SureFire Ultra Human and Mouse Total SMARCA4 Detection Kit is a sandwich immunoassay for the quantitative detection of total SMARCA4 in cellular lysates, using Alpha Technology.

Formats

• The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.
• The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.
• The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.
• The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.

AlphaLISA SureFire Ultra kits are compatible with

• Cell and tissue lysates
• Antibody modulators
• Biotherapeutic antibodies

Alpha SureFire kits can be used for

• Cellular kinase assays
• Receptor activation studies
• Screening