For research use only; not for diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Angiotensin-converting enzyme 2 (ACE2) is a human cell surface receptor that has been identified as the main point of entry for SARS-CoV-2 virions, which match and bind ACE2 with their own viral Spike protein S1 subunits. This interaction is therefore a key target in COVID-19 therapeutic research, as its inhibitors may yield a protective effect by preventing SARS-CoV-2 entry into cells. For this reason, protein-protein interaction assays suitable for ACE2-Spike inhibitor screening are relevant to current research.
The AlphaLISA detection of SARS-CoV-2 S1/ACE2 binding uses acceptor beads coupled to human ACE2 protein and Streptavidin-coated donor beads to capture the biotinylated S1-protein. Donor beads and acceptor beads come into proximity through S1 binding to ACE2. Excitation of the Donor beads provokes the release of singlet oxygen that triggers a cascade of energy transfer reactions in the Acceptor beads, resulting in a sharp emission at 615 nm (Figure 1).This assay can facilitate the screening of inhibitors of S1/ACE2 binding. A competitor of the interaction will disrupt the S1/ACE2 binding leading to a decrease of the AlphaLISA Signal.
檢測目標 | SARS-CoV2 ACE2/S1 |
---|---|
檢測技術 | Alpha |
自動化兼容 | Yes |
檢測方法 | Alpha |
實驗類型 | In vitro |
產品品牌名稱 | AlphaLISA |
運輸條件 | 藍冰 |
治療領域 | Virology |
產品尺寸 | 5,000 Assay Points |
Alpha (Amplified Luminescent Proximity Homogeneous Assay) technology is a bead-based, no-wash alternative to traditional ELISAs. Instead of detection with an HRP substrate, the Alpha assay signal is generated by the excitation of an Eu+-coated bead that has been ...
Biomarker levels were measured directly in CulturPlates-96 and-384 in a simple, fast, all-in-one-well AlphaLISA assay format. The elimination of transfer and wash steps simplifies cellular assays, reduces variability and significantly reduces hands-on time and costs associated with consumables. Int ...
The AlphaLISA® assay is a homogeneous immunoassay alternative to classical ELISA. AlphaLISA assays were originally utilized to detect analytesin cell cultures upernatants or serum/plasma samples.