The interactions and bindingof proteins are implicated in a large number of biological processes. The needfor an efficient, highly sensitive assay to study large protein interactions is increasingly important. Alpha Technology is a highly flexible, homogeneous, no-wash assay ideal for the measuremen ...

AlphaLISA technology is a powerful and versatile platform that offers highly sensitive, no-wash immunoassays using Alpha Donor and AlphaLISA Acceptor beads. In this technical note, we present the optimization of an epigenetic enzymatic assay using a full-length histone H3 substrate.

Alpha has been used to study a wide variety of interactions, including protein:protein, protein:peptide, protein:DNA, protein:RNA, protein:carbohydrate, protein:small molecule, receptor:ligand, and nuclear receptor:ligand interactions. Both cell-based and biochemical interactions have been monitored ...

Covalen modification of DNA through methylation is catalyzed by specific DNA methyltransferases (DNMTs).

Anti-mark antibodies coupled to AlphaLISA Acceptor beads or labeled with LANCE Ultra europium chelate were used for the successful optimization of robust and, sensitive epigenetic assays using histone H3-derived peptides as substrates.

In eukaryotes, the covalent modification of histones has a crucial role in chromatin architecture and plays an important part in a plethora of cellular processes, from chromatinre modeling and transcriptional regulation, to DNA repair and cell cycle control.

The AlphaLISA® assay is a homogeneous immunoassay alternative to classical ELISA. AlphaLISA assays were originally utilized to detect analytesin cell cultures upernatants or serum/plasma samples.