For research use only; not for diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
AlphaLISA® biotinylated monoclonal antibody recognizing the carboxy-terminal end of human histone H3. Broad species cross-reactivity is expected based on sequence similarity. This product is designed to be used with AlphaLISA® Epigenetics Acceptor beads to detect modified full-length histone H3 in homogeneous AlphaLISA assays.
| 自動化兼容 | Yes |
|---|---|
| 檢測方法 | Alpha |
| 實驗類型 | In vitro |
| Format | Microplates |
| 產品品牌名稱 | AlphaLISA |
| 運輸條件 | 藍冰 |
| 產品尺寸 | 2 µg |
AlphaLISA technology is a powerful and versatile platform that offers highly sensitive, no-wash immunoassays using Alpha Donor and AlphaLISA Acceptor beads. In this technical note, we present the optimization of an epigenetic enzymatic assay using a full-length histone H3 substrate.
Covalen modification of DNA through methylation is catalyzed by specific DNA methyltransferases (DNMTs).
Anti-mark antibodies coupled to AlphaLISA Acceptor beads or labeled with LANCE Ultra europium chelate were used for the successful optimization of robust and, sensitive epigenetic assays using histone H3-derived peptides as substrates.
In eukaryotes, the covalent modification of histones has a crucial role in chromatin architecture and plays an important part in a plethora of cellular processes, from chromatinre modeling and transcriptional regulation, to DNA repair and cell cycle control.
