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FCGR2B/CD32b AlphaLISA Binding Kit, 500 Assay Points

This kit is designed for the detection of binding between FCGR2B (CD32b) and human IgG Fc fragment using a homogeneous AlphaLISA® assay (no wash steps). This assay can facilitate the design and development of antibody therapeutics by using competitive binding.

For research use only; not for diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).

貨號
產品尺寸
AL3080C
500 Assay Points
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AL3080F
5,000 Assay Points
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詳細信息

This kit is designed for the detection of binding between FCGR2B (CD32b) and human IgG Fc fragment using a homogeneous AlphaLISA assay (no wash steps). This assay can facilitate the design and development of antibody therapeutics by using competitive binding.

Features:

  • No-wash steps, no separation steps
  • Ease-of-use: few addition steps, fast assay development
  • Broad range of affinities: detect strong or weak interactions, from pM to mM affinity
  • Distance: measure very large protein or antibody complexes – spanning up to 200 nm or more
  • High avidity: multiple binding sites on each bead enables use of nanomolar concentrations of antibodies or proteins, as well as use of low affinity binders

The Fc-Gamma Receptors (FCGRs) are members of immunoglobulin superfamily and play a critical role in the function of therapeutic antibodies. FCGRs are divided into three classes Fc-Gamma Receptor 1 (CD64), FCGR1; Fc-Gamma Receptor 2 (CD32), FCGR2 and Fc-Gamma receptor 3 (CD16), FCGR3. FCGR2 is expressed as two distinct forms (FCGR2A and FCGR2B) encoded by two different highly homologous genes in a cell type specific manner.

FCGR2 is a low/intermediate affinity receptor for polyvalent immune-complexed IgG. It is involved in phagocytosis, secretion of enzymes and inflammatory mediators, antibody-dependent cellular cytotoxicity (ADCC). FCGR2A delivers an activating signal upon ligand binding. In contrast, FCGR2B delivers an inhibitory signal.

The AlphaLISA detection of FCGR2B and IgG Fc fragment binding uses IgG Fc AlphaLISA Acceptor beads to capture the human FCGR2B and Streptavidin-coated Donor beads to capture the biotinylated human FCGR2B. Donor beads and Acceptor beads come into proximity through IgG Fc fragment binding to FCGR2B. Excitation of the Donor beads provokes the release of singlet oxygen that triggers a cascade of energy transfer reactions in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

規格

檢測目標 FCGR2B
檢測目標類 Fc-receptor
自動化兼容 Yes
檢測方法 Alpha
產品品牌名稱 AlphaLISA
運輸條件 藍冰
產品尺寸 500 Assay Points
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Fast, Simple, Homogeneous Assays for Characterization of Fc Receptor Binding Using AlphaLISA

Therapeutic antibodies have proven to be effective and safe treatments for various diseases, and understanding the binding of therapeutic antibodies to Fc receptors provides insights into their mechanism of action (MOA), efficacy, and safety. During early drug development, as potential therapeuti ...

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