爱游戏平台注册登录
check quantity

Human FcGR3B (CD16b) AlphaLISA Binding Kit, 5,000 Assay Points

The AlphaLISA FCGR3B (CD16b) binding kit is designed for the detection of binding between FcγR3B (CD16b) and human IgG Fc fragment using a homogeneous AlphaLISA assay (no wash steps). This assay can facilitate the design and development of antibody therapeutics using a competition assay format.

For research use only; not for diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).

貨號
產品尺寸
AL3094C
500 Assay Points
更多
AL3094F
5,000 Assay Points
更多
Request Technical Support

請輸入有效數量

請登錄以添加收藏夾。

購物車為空

詳細信息

This kit is designed for the detection of binding between FCGR3B (CD16b) and human IgG Fc fragment using a homogeneous AlphaLISA assay (no wash steps). This assay can facilitate the design and development of antibody therapeutics by using a competition assay format.

AlphaLISA features:

  • No-wash steps, no separation steps
  • Ease-of-use: few addition steps, fast assay development
  • Broad range of affinities: detect strong or weak interactions, from pM to mM affinity
  • Distance: measure very large protein or antibody complexes – spanning up to 200 nm or more
  • High avidity: multiple binding sites on each bead enables use of nanomolar concentrations of antibodies or proteins, as well as use of low affinity binders

The Fc-Gamma Receptors (FCGRs) are members of immunoglobulin superfamily and play a critical role in the function of therapeutic antibodies. FCGRs are divided into three classes Fc-Gamma Receptor 1 (CD64), FCGR1; Fc-Gamma Receptor 2 (CD32), FCGR2 and Fc-Gamma receptor 3 (CD16), FCGR3. FCGR3 is expressed as two distinct forms (FCGR3A and FCGR3B) encoded by two different highly homologous genes in a cell type specific manner. FCGR3 is a low/intermediate affinity receptor for polyvalent immune-complexed IgG. FcGR3B binds complexed or aggregated IgG and also monomeric IgG. Contrary to FCGR3A, FCGR3B is not capable to mediate antibody-dependent cytotoxicity and phagocytosis. It may serve as a trap for immune complexes in the peripheral circulation which does not activate neutrophils.

AlphaLISA detection of FCGR3B and IgG Fc fragment binding uses IgG Fc AlphaLISA® Acceptor beads and Streptavidin-coated Donor beads to capture biotinylated human FCGR3B. Donor beads and Acceptor beads come into proximity through IgG Fc fragment binding to FCGR3B. Excitation of the Donor beads provokes the release of singlet oxygen that triggers a cascade of energy transfer reactions in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

規格

自動化兼容 Yes
檢測方法 Alpha
產品品牌名稱 AlphaLISA
運輸條件 藍冰
產品尺寸 5,000 Assay Points
資源
  • 所有

產品手冊

應用文獻

Effects of 5FU and Sorafenib on Proliferation and Biomarker Expression in a Colorectal Cancer Model Using AlphaLISA and EnSight Solutions

A variety of chemotherapeutic drugs with different modes of action have been developed and tested as potential therapies for colorectal cancer. Characterizing the effects of potential drugs with different modes of action is a key part of the process.

In this application note you will learn: ...

PDF 3 MB

技術說明

Fast, Simple, Homogeneous Assays for Characterization of Fc Receptor Binding Using AlphaLISA

Therapeutic antibodies have proven to be effective and safe treatments for various diseases, and understanding the binding of therapeutic antibodies to Fc receptors provides insights into their mechanism of action (MOA), efficacy, and safety. During early drug development, as potential therapeuti ...

PDF 1 MB
Baidu
map