AlphaLISA Human and Mouse Insulin Detection Kit, 100 Assay Points

Insulin is produced in the pancreas by Beta-cells of the Langherans islets as a 110 amino acid proinsulin hormone. Proinsulin is post-translationally cleaved into two chains (peptide A of 21 amino acids and peptide B of 30 amino acids) that are covalently bonded through two disulfide bonds and released when blood glucose levels rise. After 30 minutes, the blood concentration rises from roughly 50 pmol/L to 300-400 pmol/L. Insulin regulates glucose and lipid metabolism and has been linked to several disorders, including diabetes, heart disease, and obesity.

Formats

• Our 100 assay point kit allows you to run 100 wells in 96-well format, using a 100 µL reaction volume (10 µL of sample).
• Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
• Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

Features

• No-wash steps, no separation steps
• ELISA alternative technology
• Sensitive detection
• Broad sample compatibility
• Small sample volume
• Results in less than 3 hours
• Half the time of an ELISA assay

AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.