HCV cAg AlphaLISA Detection Kit, 500 Assay Points

Formats:

• Our 100 assay point kit allows you to run 100 wells in 96-well format, using a 100 µL reaction volume (10 µL of sample).
• Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
• Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

AlphaLISA features:

• No-wash steps, no separation steps
• ELISA alternative technology
• Sensitive detection
• Broad sample compatibility
• Small sample volume
• Results in less than 3 hours
• Half the time of an ELISA assay

AlphaLISA technology allows the detection of molecules of interest in buffer, cell culture media, serum and plasma in a highly sensitive, quantitative, reproducible and user-friendly mode. In an AlphaLISA assay, a Biotinylated Anti-Analyte Antibody binds to the Streptavidin-coated Alpha Donor beads, while another Anti-Analyte Antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads provokes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

Hepatitis C virus is one of many viruses that causes liver diseases (liver inflammation and liver fibrosis) and is transmitted via blood through such means as unprotected sexual activity, blood transfusion, mother-to-infant transmission, or occupational exposure to blood. About 143 million people worldwide (2%) have been infected with hepatitis C. Chronic HCV infection often leads to liver cirrhosis and failure. The infection can be detected by HCV serology and detection of HCV antibodies. Quantitative detection of HCV cAg has been used increasingly as the most important marker for monitoring HCV titer, disease progression, and assessing antiviral treatment.